牛A-FABP与SREBP1基因的组织表达规律研究  

Gene Expression Patterns of A-FABP and SREBP1 in Cattle Tissues

作　　者：张乐[1] 彭燮[1] 刘扬[1] 付常振[1] 王洪宝[1,2] 昝林森[1,2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]国家肉牛改良中心,陕西杨凌712100

出　　处：《家畜生态学报》2014年第11期18-23,共6页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金(31272411;31000998);国家"863"计划(2013AA102505);中央高校基本科研业务费专项资金资助(2012BAD12B07)

摘　　要：A-FABP(脂肪细胞型脂肪酸结合蛋白)和SREBP1(固醇调节元件结合蛋白)是动物体内参与脂肪沉积和脂质合成的重要基因。为了研究这两种基因在牛组织中的表达规律,研究利用qRT-PCR技术检测A-FABP和SREBP1在荷斯坦牛13种组织中表达水平,并进一步比较了这两个基因在秦川牛和雪龙黑牛脂肪、肌肉和肝脏的组织中表达差异。研究结果表明A-FABP基因在脂肪组织中的表达水平远远高于其他组织,SREBP1基因在各组织中均有表达,脂肪组织的相对表达量最高。A-FABP基因在雪龙黑牛肌肉和肝脏组织中的表达量显著高于秦川牛的肌肉和肝脏(P<0.05),而在脂肪组织中,秦川牛和雪龙黑牛该基因表达量无显著差异(P>0.05)。SREBP1基因在雪龙黑牛肌肉和肝脏组织中的表达量显著高于秦川牛的肌肉和肝脏(P<0.05),而在脂肪组织中,秦川牛表达量显著高于雪龙黑牛(P<0.05)。由于A-FABP和SREBP1在脂肪中表达量最高,且它们的表达量在脂肪沉积能力不同的秦川牛和雪龙黑牛肌肉和肝脏组织中存在显著差异,故推断这两种基因在牛脂肪组织的脂质代谢过程中发挥重要作用,对脂肪的沉积和大理石花纹牛肉的形成起到一定的调控作用。To study the expression patterns of gene A-FABP （Adipocyte fatty acid-binding protein） and SREBPI（Sterol regulatory element binding protein）, two important genes involving in fat deposition and lipid synthesis, in cattle tissues, the current study detected the expression level of A-FABP gene and SREBP1 in 13 Holstein tissues with qRT-PCR and further compared the differences of their expression in the fat, muscle and liver tissues of Qinchuan and Snowdragon cattle. Results showed that the expression level of A-FABP in fat tissue was much higher than that in other tissues, and SREBP1 showed in all tissues and its relative transcript level in fat tissues ranked the highest. The mRNA levels of A-FABP in muscle and liver of Snowdragon cattle were significantly higher than those in Qinchuan cattle （P〈0.05）, while no significant difference was detected in fat between the two cattle breeds（P〈0.05）. SREBP1 in muscles and liver of Snowdragon cattle were significantly higher than those in Qinchuan cattle （P〈0.05）, while lower in fat of former than that of the latter （P〈0.05）. In conclusion, A-FABP and SREBP1 genes were detected in fat tissue the most and their expression level varied significantly between muscles and livers of Snowdragon black and Qinchuan cattle with different fat deposition capacities, indicating that these two genes effected importantly on lipid metabolism in cattle fat tissues and to a certain degree regulated the fat deposition and marbling meat formation of cattles.

关 键 词：A-FABP基因 SREBP1基因 QRT-PCR 秦川牛 雪龙黑牛 表达谱 

分 类 号：S811.6[农业科学—畜牧学]