钼镉联合胁迫对山羊肾线粒体抗氧化功能的影响  

Effect of Molybdenum Combined with Cadmium on Antioxidant Function of Kidney Mitochondria in Goat

作　　者：王琦[1] 庄煜[1] 曹华斌[1] 胡国良[1] 罗军荣[1] 顾小龙[1] 

机构地区：[1]江西农业大学动科院动物群发性疾病监测和防治研究所,江西南昌330045

出　　处：《江西农业大学学报》2014年第6期1337-1342,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金资助项目(31101863);江西省自然科学基金(2010GQN0052);江西省教育厅科学基金资助项目(GJJ10103)

摘　　要：旨在观察镉胁迫下钼对山羊肾线粒体抗氧化能力,以期探讨两者的毒理学关系。选用36头健康波尔山羊随机分成4组,每组9头,选用钼酸铵[(NH4)6Mo7O24·4H2O]、氯化镉(Cd Cl2)作为实验钼源、镉源。对照组(实验1组)灌服实验组平均剂量去离子水,实验2组、实验3组和实验4组灌服Cd 1 mg/kg(BW)同时再分别灌服Mo 15 mg/kg(BW)、30 mg/kg(BW)和45 mg/kg(BW),每组3个重复。实验期50 d,于实验第0、25、50天每组取肾组织,测定相关指标。结果表明:(1)与对照组相比,在实验第25、50天时,实验组山羊肾线粒体TAOC、SOD活性降低(P<0.01),MDA、NO、NOS、CAT含量或活性升高(P<0.05),并随着时间天数的增加,肾线粒体T-AOC、SOD活性呈降低趋势(P<0.01),而MDA、NO、NOS、CAT含量或活性呈升高趋势(P<0.05);(2)实验各组比较,当镉剂量在一定水平时,各实验组之间随着灌服钼剂量的升高,T-AOC、SOD活性呈下降趋势,MDA、NO、NOS活性或含量呈上升趋势。钼镉可导致肾线粒体抗氧化功能降低、自由基含量升高,与镉呈协同关系。This experiment was conducted to determine the effect of stress from the combination of molybdenum and cadmium on free radicals of kidney in goats and explore relationship between their toxicology. 36 healthy goats were divided into the following four groups randomly,CdCl2and [（NH4）6Mo7O24·4H2O] were selected as the source of cadmium and molybdenum.The treatments consisted of the control group（no additional Mo and Cd） ,the experimental groups 2,3 and 4,which were drenched with 15 mg of Mo ×kg-1 ×BW、30 mg of Mo ×kg-1×BW and 45 mg of Mo ×kg-1×BW each day respectively,and all the experimental groups were drenched with 1 mg of Cd ×kg-1×BW each day as well.The experimental period was 50 days,renal tissue samples were obtained on the 0 day,the 25th day and the 50th day by killing 3 goats from each group respectively,then the related indexes were determined.The results were as follows：compared with the control group,the levels of MDA,NO,NOS in each group were significantly higher than those in the control group,while the levels of T-AOC,SODwere lower （P〈0.05）;in addition,as the experimental time elapsed,the levels of MDA,NO,NOS in each experimental group showed an increasing trend and the levels of T-AOC,SOD showed an decreasing trend.In theexperimental groups （except control group）：with the increasing of molybdenum dosage,the levels of T-AOC,T-SOD decreased while those of MDA,NO and NOS increased when cadmium was at the same level.（Conclusion） The results indicated that under the molybdenosis and cadmium poisoning in goat,cadmium and molybdenum had a synergetic effect on kidney mitochondria antioxidant function.

关 键 词：山羊 镉 钼 自由基代谢 

分 类 号：S827.211[农业科学—畜牧学]