雷公藤内酯醇对高糖时人肾小球系膜细胞MC P-1表达的影响  

Effect of Triptolide on the High Glucose-induced Expression of Monocyte Chemoattractant Pro-tein-1 in Human Mesangial Cells

作　　者：莫建勋[1] 孙文波[1] 蒙雯雯[1] 叶烨[1] 唐盛发[1] 莫剑锋[1] 陈植涛[1] 莫春荣[1] 杨灵红[1] 

机构地区：[1]中国人民解放军一八一医院内分泌科,广西桂林541002

出　　处：《医学临床研究》2014年第12期2392-2394,2398,共4页Journal of Clinical Research

摘　　要：目的探讨高糖及雷公藤内酯醇（TP）对人肾小球系膜细胞单核细胞趋化蛋白-1（MCP-1）表达的影响。方法将培养的人肾小球系膜细胞分为6组：正常对照组（5 mmol/L 葡萄糖）、高糖组（30 mmol/L 葡萄糖）、甘露醇组（5 mmol/L 葡萄糖＋25 mmol/L甘露醇）、TP甲组（30 mmol/L 葡萄糖＋5μg/L TP）、TP 乙组（30 mmol/L 葡萄糖＋10μg/L TP）、TP丙组（30mmol/L 葡萄糖＋15μg/L TP），分别在24 h、48 h、72 h采用 RT-PCR法检测TP对高糖条件下系膜细胞内 MCP-1 mRNA 及蛋白表达的影响。结果高糖组人肾小球系膜细胞 MCP-1 mRNA及蛋白较正常对照组增加，且差异有显著性（P＜ 0.01）；TP各组比高糖组有明显下降（P＜ 0.01）；不同浓度的TP对高糖诱导的系膜细胞 MCP-1的表达抑制程度不同（P ＜ 0.05）。结论 TP可抑制高糖诱导的人肾小球系膜细胞 MCP-1的表达，且呈时间、剂量依赖关系，其可能对延缓糖尿病肾病中肾小球硬化有一定作用。Obj ective]To explore the effect of high glucose and triptolide（TP）on the expression of mono-cyte chemoattractant protein-1（MCP-1）in human mesangial cells（HMCs）.[Methods]The cultured human HMCs were divided into normal control group（5mmol/l glucose）,high glucose group（30mmol/l glucose）, mannitol group（5mmol/l glucose with 25mmol/l mannitol）,TP A groups（30mmol/l glucose with 5ug/L TP）, TP B group（30mmol/L glucose with 10ug/L TP）and TP C group（30mmol/L glucose with 15ug/L TP）.The effect of TP on the mRNA and protein expression of MCP-1 in HMCs induced by high glucose at 24h,48h and 72h were measured by RT-PCR.[Results]Compared with normal control group,the mRNA and protein ex-pression of MCP-1 in HMCS of high glucose group were increased,and there was significant difference（P〈 0.01）,but those in TP groups were obviously decreased（P〈 0.01）.The effect of different concentrations of TP in inhibiting the expression of MCP-1 of HMCs induced by high glucose was varied（P〈 0.05）.[Conclu-sion]TP can inhibit the expression of MCP-1 in HMCs induced by high glucose in time and dose-dependent manners,and may be helpful in delaying the development and progression of glomerular sclerosis of diabetic nephropathy.

关 键 词：雷公藤内酯/药理学 糖尿病肾病 趋化因子CCL2 肾小球系膜细胞 

分 类 号：R587.24[医药卫生—内分泌]