P73基因多态性与非小细胞肺癌遗传易感性分析  

p73 Gene Polymorphism and Genetic Susceptibility to Non-Small Lung Cancer

作　　者：王霜霜[1] 董林利 汤建新[1] 李文[1] 

机构地区：[1]湖南工业大学绿色包装与生物纳米技术应用重点实验室,湖南株洲412007

出　　处：《湖南工业大学学报》2014年第6期13-16,共4页Journal of Hunan University of Technology

基　　金：国家自然科学基金资助项目(61171061);湖南省自然科学青年基金资助项目(12JJ4082;14JJ2049);湖南工业大学自然科学研究基金资助项目(2013HZX04);湖南省教育厅科学研究基金资助项目(14B050)

摘　　要：采用两对引物-聚合酶链反应（PCR-CTPP）方法分别对100例非小细胞肺癌患者和120例健康对照者进行p73基因多态性检测,DNA测序验证基因分型结果,对p73基因多态性与非小细胞肺癌的相关性进行分析,探讨湖南省地区人群中p73基因多态性与非小细胞肺癌遗传易感性的关系。试验结果表明,PCRCTPP法和测序得到的基因分型结果完全吻合,100例肺癌患者的p73基因型分布如下：GC/GC型60例（60%）、GC/AT型36例（36%）、AT/AT型4例（4%）,与携带p73 GC/GC基因型者比较,携带AT/AT基因型者的肺癌患病风险增加3.4倍（OR=3.387,95%CI=1.055-10.875,P=0.032）,而携带GC/AT基因型者肺癌风险也呈增加趋势,但是无显著性差异（OR=1.183,95%CI=0.672-2.082,P=0.561）。p73基因多态性可能与湖南省地区人群非小细胞肺癌遗传易感性相关。Used PCR-CTPP to detect the p73 gene polymorphism among 100 patients with NSCLC and 120 healthy controls respectively and DNA direct sequencing verified the results of genotypes. Analyzed the association between p73 gene polymorphism and NSCLC, and explored the correlation between p73 gene polymorphism and susceptibility to NSCLC in the Hunan population. The experiment results showed that the results of PCR-CTPP and the sequencing genotyping results were consistent, p73 genotype distribution in 100 lung cancer patients are as follows： GC/GC 60 cases（60%）, GC/AT36 cases（36%）, AT/AT 4 cases（4%）. Compared with the carriers of GC/GC genotype, the carriers of AT/AT genotype significantly increased 3.4 times the risk of NSCLC（OR=3.387, 95%CI= 1.055-10.875, P=0.032）, also the carriers of GC/AT genotype tended to increase the risk of NSCLC, but had no significantly difference（OR=1.183, 95%CI= 0.672-2.082,P=0.561）. The p73 gene polymorphism may have the relation with the susceptibility to NSCLC in the Hunan population.

关 键 词：NSCLC p73基因多态性 PCR-CTPP法 

分 类 号：Q75[生物学—分子生物学]