肾安冲剂对阿霉素肾病大鼠肾组织TGF-β_1、α-SMΑ、CTGF及Col-Ⅰ表达的影响  

Effects of Shen An granules on expression of TGF-β_1,α-SMΑ,CTGF,and Col-I in rats with adriamycin nephropathy

作　　者：符逢春[1] 刘百祥[2] 简维雄[3] 刘彬[1] 林辉[1] 刘起胜[1] 

机构地区：[1]湖南中医药高等专科学校,湖南株洲412012 [2]湖南省人民医院,湖南长沙410005 [3]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2014年第12期134-135,146,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药管理局重点科研项目(编号:2009009);湖南省教育厅青年科研项目(编号:08B059)

摘　　要：目的:探讨肾安冲剂对阿霉素肾病大鼠肾间质纤维化的影响。方法:将150只雄性SD大鼠随机分为正常组、模型组、肾安冲剂小剂量组和肾安冲剂大剂量组、泼尼松加缬沙坦组。对除正常组外的其余组大鼠尾静脉注射阿霉素建立阿霉素肾病模型。各组大鼠分别于造模后8、12、16周末分批处死,免疫组化法检测肾组织中TGF-β1、α-SMA、CTGF和Col-Ⅰ的表达。结果:各治疗组大鼠肾组织中TGF-β1、Col-Ⅰ、CTGF和α-SMA的表达均较模型组明显减少(P<0.05),且以肾安冲剂大剂量组最显著。结论:肾安冲剂可能通过下调肾组织TGF-β1、Col-Ⅰ、CTGF和α-SMΑ的表达而抑制ECM的合成,从而达到延缓阿霉素肾病大鼠肾小管间质纤维化的目的。Objective：To investigate the effects of Shen An granules on the renal interstitial fibrosis of rats with adriamyein nephropathy. Metbods：A total of 150 male Sprague- Dawley rats were randomly divided into normal group ,model group, low- and high -dose Shen An granule groups, and prednisone combined with valsartan group. All the groups except the normal group were intravenously injected with adriamycin to establish an adriamycin nephropathy model. The rats in each group were killed 8,12, and 16 weeks after the establishment of model. Immunohistochemistry was used to measure the expression of TGF - β1,α-SMA, CTGF, and Col -I in renal tissues. Results：Compared with the model group, all the other treatment groups had significantly lower expression of TGF- β1,α- SMA, CTGF, and Col- I in renal tissues at different time points （P 〈 0. 05 ）, with high -dose Shen An granules group having the most significant decrease. Conclusion：Shen An granules can inhibit the synthesis of extra cellular matrix by down -regulating the expression of TGF -β1,α -SMA, CTGF, and Col -I in renal tissues, thus delaying renal tubule interstitial fibrosis in rats with adriamycin nephropathy.

关 键 词：肾安冲剂 阿霉素肾病 TGF-β1 α-SMA、CTGF、Col-Ⅰ 实验研究 

分 类 号：R285.5[医药卫生—中药学]