ATP荧光检测法与细菌定量检测法现场细菌污染检测相关性研究  

STUDY ON CORRELATION OF BACTERIAL CONTAMINATION DETECTION ON THE SPOT OF QUANTITATIVE METHOD AND ATP FLUORESCENCE ASSAY

作　　者：王绍鑫[1] 周艳琴[1] 罗剑峰[2] 李凌雁[1] 成慜[1] 

机构地区：[1]上海市卫生局卫生监督所,上海200031 [2]复旦大学

出　　处：《中国消毒学杂志》2014年第12期1285-1287,共3页Chinese Journal of Disinfection

摘　　要：目的研究ATP生物荧光检测法与细菌定量检测法对现场环境物体表面细菌负载检测结果相关性及数学模型。方法通过现场采样与检测方法,对两种检测技术结果进行统计分析和评价。结果 ATP荧光检测法与常规细菌定量检测法检测结果 Peason相关系数为0.851,二次方模型y=0.804-0.095x+0.309 x2(R=0.785)拟合度较高。结论 ATP荧光检测法与常规细菌定量检测法在现场物体表面细菌负载检测中结果具有较好相关性,其检测结果具有一定的可靠性。Objective To study the correlation and the mathematic model of ATP fluorescence assay and bacterial quantitative detection method for the bacteria load on the object surface on the spot. Methods The field sampling and detection methods were used to carry out statistical analysis and evaluation of the two detection results. Results The Peason correlation coefficient of ATP fluorescence test and result of bacteria counting method was 0. 851. The fitting degree quadratic model y = 0. 804- 0. 095 x ＋ 0. 309 x2（ R = 0. 785） was higher. Conclusion Results of the ATP fluorescence assay and the lab bacteria counting method for the bacteria load on the object surface on the spot have a good correlation. Results Results of these detections are reliable.

关 键 词：ATP生物荧光法 细菌定量检测法 细菌污染 相关性 

分 类 号：R181.2[医药卫生—流行病学]