铯137辐照对库存红细胞表面分子CD35、CD47的影响  

EFFECT OF ^(137)Cs RADIATION ON CD35 AND CD47 OF STORAGE ERYTHROCYTES

作　　者：赵学涛[1] 杨从容[1] 任晓亮[1] 李明[1] 解晓元[1] 巨红妹[1] 

机构地区：[1]河北医科大学第四医院输血科,河北石家庄050011

出　　处：《河北医科大学学报》2014年第12期1414-1416,共3页Journal of Hebei Medical University

基　　金：河北省卫生厅医学科学研究重点课题(20090532)

摘　　要：目的探讨铯137(^(137)Cs)辐照对库存红细胞表面分子红细胞1型补体受体(complement receptor type1,CR1 or CD35)及CD47分子的影响。方法采集60例健康献血者静脉血,制备去白细胞的悬浮红细胞,并将其分为2组各30例,未辐照组常规4℃保存,辐照组采用25Gy^(137)Cs辐照后4℃保存。分别于第0、7、14、28、35天收集2mL,样本。采用直标法流式细胞术检测红细胞表面分子CD35、CD47。结果辐照组和未辐照组红细胞CD35、CD47的表达均随着4℃保存时间的延长而减少,2组的时点间以及组间·时点间交互作用比较差异均有统计学意义(P<0.01),但组间差异无统计学意义(P>0.05)。结论 25Gy^(137)Csγ射线辐照对保存期内红细胞CD35、CD47表面分子表达无明显影响。Objective To investigate the effects of 25Gy 137 Cs radiation on the red cell surface molecule complement receptor type 1（CD35）or CD35 and CD47 in storage erythrocytes. Methods The vein blood samples of healthy donors were collected,leukocyte-depleted suspended erythrocytes were prepared.The specimens of 60 cases were randomly divided into two groups with 30 cases in each group and stored at 4℃,namely non-irradiation group （control group）and irradiation group.The suspended erythrocytes （2mL）were respectively collected on the 0,7th, 14th,21st,28th,and 35th day after the storage of two groups.The quantity of CD35 and CD47 of erythrocytes were assessed by direct calibration method using flow cytometry.Results The quantity of erythrocytes CD35 and CD47 of non-irradiated group and irradiation group decreased during storage at 4℃.The difference had statistical significance in different storage times and interactions between groups and time （P 〈 0.01 ）.However,intergroup difference was of no significance （P 〉0.05）.Conclusion 25Gy 137 Cs radiation does not have significant effects on the CD35 and CD47 expression.

关 键 词：红细胞 Γ射线 受体 补体 抗原 CD47 

分 类 号：R331.122[医药卫生—人体生理学]