淮南麻黄鸡CRBP2基因第3外显子PCR-SSCP多态性及其与蛋品质的相关分析  

PCR-SSCP Polymorphism of Exon 3 of CRBP2 Gene and Correlation Analysis with Egg Quality in Huainan Partridge Chicken

作　　者：李俊营[1] 詹凯[1] 柏如东[2] 刘伟[1] 刘盛南[1] 韩涛 

机构地区：[1]安徽省农业科学院畜牧兽医研究所,合肥230031 [2]安徽农业大学生命科学学院,合肥230036 [3]安徽皖西麻黄鸡禽业有限公司,六安237400

出　　处：《吉林农业大学学报》2014年第6期697-700,706,共5页Journal of Jilin Agricultural University

基　　金：国家蛋鸡产业技术体系(CARS-41-K19);安徽省农业科学院院长青年创新基金项目(13B0425)

摘　　要：利用PCR-SSCP和DNA测序技术对淮南麻黄鸡CRBP2基因第3外显子的多态性进行了检测,并分析其与蛋品质的相关性。结果表明:在淮南麻黄鸡样本群中CRBP2基因第3外显子存在C/T的突变位点,共检测到3种基因型(CC、CT和TT),C和T等位基因的频率分别为0.45和0.55,多态信息含量和杂合度分别为0.37和0.47,表明该位点处于中度多态。χ2适合性检验表明淮南麻黄鸡样本群在该位点偏离Hardy-Weinberg平衡状态(P<0.01)。淮南麻黄鸡CRBP2基因第3外显子各基因型间开产日龄、蛋形指数、蛋壳厚度、蛋壳强度、蛋黄色泽和蛋黄比率差异不显著(P>0.05),CC基因型的开产蛋重和30周龄蛋重分别比TT基因型高1.66%和2.60%(P<0.05),TT基因型的哈氏单位显著高于CC和CT基因型(P<0.05)。The polymorphism of exon 3 of CRBP2 gene in Huainan partridge chicken and its association with egg quality were investigated by PCR-SSCP and DNA sequencing technologies. The results showed that a mutation （ C/T） was found in the exon 3 of CRBP2 gene, three genotypes （ CC, CT and TT） were detected in Huainan partridge chicken, and the frequencies of allele C and T, polymorphism information content and heterozygosity were 045, 055, 037 and 047, respectively, which revealed a moderate polymorphism in this site. Statistical results of χ2 test indicated that this site did not fit HardyWeinberg equilibrium （ P0.05 ） , egg weight at first laying and egg weight at 30th week with genotype CC were 166% and 260% higher than those with genotype TT （ P〈0.05） , respectively. The value of Haugh unit with genotype TT was higher than that with genotype CC and CT （ P〈0.05） .

关 键 词：淮南麻黄鸡 CRBP2基因 多态性 蛋品质 

分 类 号：S813.2[农业科学—畜牧学]