漆酶/组氨酸和漆酶/谷氨酸改善二次纤维性能的比较  

The Comparison between Laccase/Histidine and Laccase/Glutamic Acid to Improve the Performance of OCC

作　　者：杨青[1] 万金泉[1,2] 陈杨梅[1,3] 马邕文[1,2] 王艳[2] 黄明智[4] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]华南理工大学环境与能源学院,广东广州510006 [3]华南理工大学化学与化工学院,广东广州510640 [4]中山大学水资源与环境学院,广东广州510275

出　　处：《造纸科学与技术》2014年第6期89-93,共5页Paper Science & Technology

基　　金：国家自然科学基金项目(31170551);教育部博士点基金项目(20110172110015);广东省高校优秀青年创新人才培养计划项目(LYM10014);制浆造纸工程国家重点实验室开放基金项目(201306);中央高校基本科研业务费专项资金项目(2014ZB0011)

摘　　要：本实验通过改变漆酶用量、反应时间、p H、介体用量等,比较经漆酶/组氨酸和漆酶/谷氨酸改性后二次纤维抗张指数和撕裂指数的变化。实验表明:漆酶/组氨酸处理OCC浆的最佳反应条件为:漆酶用量1U/g,反应时间8h,p H值4.5,组氨酸用量1%;漆酶/谷氨酸处理OCC浆的最佳反应条件为:漆酶用量1.5 U/g,反应时间8h,p H值5,谷氨酸用量2%。实验数据显示在最佳反应条件下漆酶/组氨酸比漆酶/谷氨酸处理的抗张指数高出10%,撕裂指数高出9%,并且漆酶/谷氨酸处理的比空白样的抗张指数提高21%,撕裂指数提高18%。从经济效益出发,漆酶/谷氨酸比漆酶/组氨酸更适合用来改善二次纤维的性能。In the experiment,we change the dosage of laccase, time,pH, dosage of mediators, for comparing the pa- per tensile index and tear index after modification. Experiments show that laccase/histidine optimum reaction con- ditions are： paint dosage of enzymel U . g-1, reaction time 8 h, pH value 4.5, histidine dosage 1%. Laccase/ glutamic acid optimum reaction conditions are ： the dosage of laccase 1.5 U . g- 1, reaction time 8 h, pH 5 , glu- tamic acid dosage of 2%. From this experiment we can know that,at optimum reaction conditions, lacease/histi- dine treated tensile index was 10% higher than that of laccase/glutamic acid, tear index increased by 9% , and laccase/glutamic acid treated tensile index was 21% higher than that of control pulp, tear index increased by 18%. Taking the economic benefits into account, laccase/glutamic acid can take the place of laccase/histidine used to improve the performance of the secondary fiber.

关 键 词：漆酶 组氨酸 谷氨酸 抗张指数 撕裂指数 

分 类 号：TS724[轻工技术与工程—制浆造纸工程]