两种含1,3,4-噻二唑α-氨基膦酸酯与蛋白质弱相互作用的ESI-MS研究  

Study on Non-covalent Interactions Between Two α-Aminophosphonates Containing 1,3,4-Thiadiazole and Proteins by ESI-MS

作　　者：张宇虹[1] 韩国胜[1] 武现丽[1] 高伟霞[1] 廖新成[1] 

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《郑州大学学报（理学版）》2014年第4期73-77,共5页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目;编号21004055

摘　　要：用ESI-MS研究了两种含1,3,4-噻二唑α-氨基膦酸酯与蛋白质,包括溶菌酶、胰岛素和细胞色素c之间的非共价相互作用.结果表明,这两种含1,3,4-噻二唑α-氨基膦酸酯能与多种蛋白质形成非共价复合物,并且使溶液中蛋白质分子构象趋于收缩.对于同一种蛋白质,底物分子结构的细微差别会形成稳定性和计量比均有差别的复合物.对于同一种底物与不同蛋白质作用时,形成的复合物比例和稳定性有较大的差别.The non-covalent interactions between two α-aminophosphonates containing 1,3,4-thiadiazole and three kinds of proteins,such as lysozyme,bovine insulin and cytochrome c,were studied by eletrospray ionization-mass spectroscopy（ ESI-MS）. The results showed that the two new compounds could easily form non-covalent complexes with proteins. The substrates could contract the protein configuration in solution. A fine distinction between chemistry structures of substrates could result in different stability and stoichiometric proportion of complexes for one protein. It showed that there may be much difference among these results when one substrate reacted with different proteins.

关 键 词：ESI-MS 1 3 4-噻二唑 Α-氨基膦酸酯 蛋白质 非共价相互作用 

分 类 号：O657.61[理学—分析化学]