离子色谱法测定1,4-丁二磺酸腺苷蛋氨酸中1,4-丁二磺酸的含量  

Ion Chromatography Determination of 1,4-Butanedisulfonate in Ademetionine 1,4-Butanedisulfonate

作　　者：朱永琴[1] 景爱华[2] 王雪芹[1] 

机构地区：[1]河南省食品药品检验所,河南郑州450003 [2]河南科技大学医学技术与工程学院,河南洛阳471003

出　　处：《郑州大学学报（理学版）》2014年第4期78-80,100,共4页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目;编号81071230

摘　　要：建立测定1,4-丁二磺酸腺苷蛋氨酸中1,4-丁二磺酸含量的离子色谱方法.采用Ion Pac^（R） AS14（250 mm×4 mm）色谱柱,以3.5 mmol/L Na2CO3和1.0 mmol/L Na HCO3为流动相,流速为2.0 m L/min,结果表明,1,4-丁二磺酸在5.115~204.6 mg/L（r=0.999 7）范围内有良好的线性响应关系,平均回收率为99.4%,RSD为0.8%,最低检出限为1.0 ng.本法可准确测定1,4-丁二磺酸腺苷蛋氨酸中1,4-丁二磺酸的含量,有效控制其质量.A suppressed conductivity ion chromatography method for determination of 1,4-butanedisulfonate in ademetionine 1,4-butanedisulfonate was established. Chromatographic separation was achieved on an Ion Pac^（R）AS14（ 250 mm × 4 mm） column. The elution was consisted of 3. 5 mmol / L Na2CO3 and1.0 mmol / L NaHCO3. The flow rate was 2. 0 m L / min. The results showed that a good linearity for the content of 1,4-butanedisulfonate was found in the range of 5. 115 ~ 204. 6 mg / L with a correlation coefficient of 0. 999 7. The average recovery was 99. 4 %（ RSD = 0. 8%,n = 9）,the minimum detection limit was 1. 0 ng. The method could accurately detect 1,4-butanedisulfonate in ademetionine 1,4-butanedisulfonate,and could be used as an effective methed for the quality control of ademetionine 1,4-butanedisulfonate.

关 键 词：离子色谱 1 4-丁二磺酸腺苷蛋氨酸 1 4-丁二磺酸 

分 类 号：O657.7[理学—分析化学]