大豆再生相关基因GmWUS的克隆及表达分析  

Molecular cloning and expression of GmWUS related to regeneration in soybean

作　　者：周扬[1] 刘薇[1] 孙石[1] 韩天富[1] 侯文胜[1] 

机构地区：[1]中国农业科学院作物科学研究所,农业部北京大豆生物学重点实验室,北京100081

出　　处：《中国油料作物学报》2014年第6期707-712,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家转基因生物新品种培育重大科技专项(2013ZX08010-004);中国农业科学院科技创新工程

摘　　要：WUS基因在植物再生过程中具有重要作用。为初步了解GmWUS的功能,基于已公布的大豆基因组数据对该基因进行了相关生物信息学分析,表明GmWUS和At WUS蛋白在酸性区域存在较大差异,而其他结构域较保守。从大豆茎尖克隆到GmWUSa的全长序列,该基因亚细胞表达定位于细胞核,表明它可能是一种同源异型域转录因子。q PCR检测种子萌发过程中子叶GmWUS的表达变化,发现GmWUSa和GmWUSb表达量均随萌发天数上升,但GmWUSa在后期表达量下降。丛生芽诱导期间,子叶节处GmWUS的表达呈现出一种先增后减的近似周期规律,表明GmWUS可能参与了丛生芽的分化和再生。比较GmWUSa和GmWUSb的表达变化趋势,推测两基因的功能可能在时间上互补。本文的结果有助于研究GmWUS在大豆再生过程的作用,解释丛生芽的形成机制和作为过表达GmWUS基因增强大豆再生能力、提高转化效率的依据。WUS plays an essential role in plant regeneration. To preliminarily understand the function of Gm- WUS, the related bioinformatics analysis was conducted based on the soybean database.. GmWUS and AtWUS proteins were different in the acidic region, while other domains were conservative. Meanwhile, GmWUSa full -length sequence had been cloned. The protein mainly localized in the nucleus, showing that it might be a homeodomain transcription factor. The expression of GmWUS in cotyledons had been detected by qPCR during seeds germination. The results showed that both GmWUSa and GmWUSb expression levels kept rising, but GmWUSa expression decreased in the late stage. GmWUS expression in cotyledon node during cluster buds induction shown an approximate periodic pattern, indicating that GmWUS involved cluster buds differentiation and regeneration. Comparing the ex- pression of GmWUSa with GmWUSb, they might play a temporal complementary in their functions. The results in this study will help to investigate roles of GmWUS in soybean regeneration, to explain the mechanism of cluster buds formation, and to promote soybean regenerative capacity so as to improve the genetic transformation efficiency by overexpressing this gene.

关 键 词：大豆再生 丛生芽 农杆菌介导转化法 WUS基因 同源异型结构域 基因表达 

分 类 号：S565.103[农业科学—作物学]