干旱胁迫下大豆抗旱突变体M18苗期生长和生理特性  

Growth and physiological characteristics of seedlings of soybean drought-resistant mutant M18 under drought stress

作　　者：莫金钢[1,2] 马建[1] 沈勇[2] 张丽辉[2] 曲静[1] 王丕武[1] 

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]长春师范大学生命科学学院,吉林长春130032

出　　处：《中国油料作物学报》2014年第6期770-776,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(30971805)

摘　　要：为了考察大豆抗旱突变体M18苗期植株的生长状况和生理特性,以其野生型亲本大豆吉农18号(JN18)为对照,于苗期采用不同浓度(0%、5%、15%、25%)PEG-6000模拟干旱胁迫处理,测定其形态性状和生理生化指标。结果表明:随着PEG浓度的增加,M18和JN18植株的主根长、一级侧根数、侧根总长度、叶绿素含量等指标呈先增加后减少的趋势,而株高、地上部鲜重和干重及RWC等指标逐渐减小,根冠比、Pro含量、MDA含量、SOD活性和POD活性等指标逐渐增加;在同一浓度PEG处理下,两者的根干重和SOD活性均呈极显著性差异(P<0.01),M18比JN18具有较强的生长优势和较高的叶片保水能力、渗透调节能力和酶促抗氧化能力。根体积、株高、冠干重、脯氨酸含量等具有显著性差异的指标均可作为大豆苗期抗旱性鉴定的主要指标。To evaluate the growth and physiological characteristics of droughtresistant soybean mutant M18, 0% , 5% , 15% , 25% of PEG-6000 were applied at soybean seedling stage as drought stress conditions. JN18, a wild - type line was selected as control. The results showed that taproot length, number of lateral roots, total length of lateral roots, root volume, root dry weight of both M18 and JN18 increased initially and then decreased with the increasing of PEG concentration ; the plant height, crown fresh weight, crown dry weight and RWC gradually decreased; the ratio of root/shoot, Pro content, MDA content, SOD activity and POD activity gradually increased. There was highly significant difference between dry root weight, SOD activity of M18 and J18. M18 had higher water retention, osmotic adjustment ability and enzymatic antioxidant capacity than those of J18 under the same drought stress. These morphological indexes and physiological characteristics including root volume, plant height, crown dry weight, and Pro content, could be used to evaluate drought resistance of soybean.

关 键 词：大豆 突变体 形态指标 生理特性 抗旱性 

分 类 号：S565.103[农业科学—作物学]