菊苣酸诱导人白血病细胞HL-60凋亡作用及机制研究  

Apoptotic effect of chicoric acid on human leukemia cell line HL-60

作　　者：陈瑞奇[1,2] 罗桂芳 张宗利 陈文丽 曾光尧[4] 信红亚[1] 

机构地区：[1]中南大学湘雅医院血液科,长沙410008 [2]中南大学湘雅医学院临床医学系,长沙410013 [3]湖南省药物安全评价研究中心,长沙410331 [4]中南大学药学院,长沙410013

出　　处：《中南药学》2014年第12期1208-1210,1250,共4页Central South Pharmacy

基　　金：湖南省科技计划项目(No.2011SK3225)

摘　　要：目的观察菊苣酸诱导人急性髓系白血病细胞株HL-60凋亡的作用并初步探讨其机制。方法培养HL-60细胞,分别给予终浓度为10-100μmol·L^-1的菊苣酸48 h,检测细胞活性和凋亡、Caspase-3活性以及Bcl-2蛋白表达水平。结果终浓度为10-100μmol·L^-1的菊苣酸能呈浓度依赖性降低HL-60细胞增殖活性和增加凋亡,增强Caspase-3活性和下调Bcl-2蛋白表达。结论菊苣酸具有抑制白血病细胞株HL-60增殖活性和诱导其凋亡作用,其机制与降低抗凋亡蛋白Bcl-2表达及增强Caspase-3活性有关。Objective To observe the apoptotic effect of chicoric acid on human leukemia cell line HL-60. MethodsHL-60 cells were cultured and incubated wi th chicoric acid（final concentration： 10- 100 μmol·L^- 1） for 48 h. The cell viability and apoptosis, Caspase-3 activity and expression of Bcl-2 protein were determined. Results Chicoric acid（final concentration： 10- 100 μmol·L^- 1） concentration-dependently decreased the cell proliferative viability and increased the apoptosis in HL-60 cells. The enhance of Caspase-3 activity and down-regulation of Bcl-2 protein expression were observed in HL-60 cells treated with chicoric acid. Conclusion Chicoric acid can inhibit the viability and induce apoptosis in HL-60 cells, which may be related to the expression down-regulation of anti-apoptotic protein Bcl-2 and increase of Caspase-3 activity.

关 键 词：菊苣酸 白血病细胞 凋亡 

分 类 号：R285[医药卫生—中药学]