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作 者:刘进[1] 李清[1] 张宇[1] 任春元[1] 杨贤莉[1] 姚晓云[1] 王嘉宇[1]
机构地区:[1]沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室
出 处:《核农学报》2014年第12期2153-2158,共6页Journal of Nuclear Agricultural Sciences
基 金:辽宁省高等学校优秀人才支持计划资助(LJQ2013075)
摘 要:为了分析粒形性状的分子遗传机理,本研究以Sasanishiki/Habataki//Sasanishiki///Sasanishiki回交重组自交系群体为试验材料,通过3年的重复试验,采用完备复合区间作图法(ICIM)对粒长、粒宽、粒厚等4个粒形性状进行数量性状定位。共检测到20个粒形QTLs,分布在第2、3、4、5、7、11和12号染色体上,单个性状QTL数目在4~7之间。研究发现有4个QTL-q GW3a、q GW3b、q LWR3和q GT2能在3年中能被重复检测到;有5个QTL-q GL2、q GL7、q GW2、q GW12和q LWR2被检测到2次。这些高重复性的稳定QTL可用于分子辅助选择育种。粒形QTL在第2、3(2)和7号染色体上呈簇分布。此外,试验检测到3个新的QTL位点GT3、GT7和GT11,这反映出粒形遗传的复杂性。本研究结果将为水稻粒形性状分子育种提供理论基础。To further understand how the molecular genetic mechanism of rice grain type traits. In this study,based on three years data, a backcross recombinant inbred lines(BILs) derived from Sasanisiki/Habataki// Sasanisiki// /Sasanishiki and its linkage map including 236 molecular markers were used for analyzing and mapping grain type QTLs by ICIM method. A total of 20 QTLs were identified on chromosome 2,3,4,5,7,11 and 12. Each trait had 4 to 7QTLs. q GW3 a,q GW3 b,q LWR3 and q GT2 were detected in all three years,and q GL2,q GL7,q GW2,q GW12 and q LWR2 were detected in two years. These constitutive QTLs provide references for molecular assistant select breeding in rice. Four QTLs clusters were identified on chromosome 2、3(2) and 7. Meanwhile,we also detected several new QTL(q GT3,q GT7,q GT11). These data indicated the genetic basis of shape traits is complexity. The result of this work will lay the foundation of the further study of the mechanism for molecular breeding of rice grain trats.
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