花生中胁迫相关基因AhDHN1的克隆及非生物胁迫下表达分析  

Cloning of a Dehydrin Gene AhDHN1 and Its Expression Analysis During Abiotic Stresses in Peanut

作　　者：陈娜[1] 胡冬青 潘丽娟[1] 迟晓元[1,3] 陈明娜[1] 王通[1] 王冕[1] 杨珍[1] 禹山林[1] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]青岛市出入境检验检疫局,山东青岛266001 [3]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《核农学报》2014年第12期2159-2166,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家花生产业技术体系项目(CARS-14);山东省自然基金项目(ZR2011CQ036;ZR2012CQ031);国家自然科学基金项目(31000728;31100205;31200211);青岛市科技计划应用基础研究项目[12-1-4-11-(2)-jch];农业部油料作物生物学与遗传育种重点实验室开放课题基金(2014010)

摘　　要：低温、高盐和干旱胁迫严重影响作物的生长和产量。为了挖掘花生逆境胁迫相关功能基因,本研究以花生品种花育33号为试验材料,根据c DNA文库中已知的脱水素(dehydrin,Dhn)基因全长序列设计引物,通过RT-PCR克隆到该基因,并命名为AhDHN1。该基因编码框为384bp,编码128个氨基酸。序列分析表明该氨基酸序列含有两个保守的富含赖氨酸片段和一个保守的富含丝氨酸片段,表明该蛋白为K2S型脱水素。荧光定量PCR结果显示,AhDHN1基因在花生的叶片和根中对低温没有明显响应,对高盐和干旱胁迫则有明显响应,说明该基因可能参与了花生对高盐和干旱胁迫的适应性调控。本研究为阐明花生抗逆分子机理提供了理论基础,为花生抗逆分子育种提供了新的基因资源。Crop growth and yield are strongly influenced by abiotic stresses such as drought,salt and cold. To screen functional genes related to abiotic stress regulation of peanut,we cloned the full length of dehydrin gene Ah DHN1 from peanut（Arachis hypogaea L. cultivar ‘Huayu33 ＇） through RT-PCR method. The open reading frame of Ah DHN1 is384 bp in length and encodes 128 amino acids. Sequence analysis indicated that Ah DHN1 contained two K-segments and one S-segment,which indicated that Ah DHN1 protein belonged to K2 S type dehydrin. The expression analysis of Ah DHN1 under various abiotic stresses was detected using real time RT-PCR. The results showed the expression of Ah DHN1 had no obvious change under cold stress in peanut leaves and roots. However,Ah DHN1 was induced distinctly during salt and drought conditions in either leaves or roots. These results suggested that Ah DHN1 may participate in the salt and drought stress regulation of peanut. This study provides a theoretical basis for elucidating the resistance molecular mechanism in peanut and provides new gene resources for molecular breeding of peanut.

关 键 词：花生 脱水素 表达分析 非生物胁迫 

分 类 号：S565.2[农业科学—作物学]