稳定同位素碳、氮在冬虫夏草原产地鉴别中的应用  

Application of nitrogen and carbon stable isotope in Ophicordyceps sinensis origin identification

作　　者：李辉[1] 姚粟[1] 刘洋[1] 白飞荣 扎西.才吉 程池[1] 

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100015 [2]玉树藏族自治州三江源药业有限公司,青海玉树810003

出　　处：《食品与发酵工业》2014年第7期173-176,共4页Food and Fermentation Industries

基　　金：青海省科技计划项目(2012-N-142;2012-N-153)

摘　　要：利用同位素比值质谱仪(IRMS)测定了来自我国青海省、云南省、四川省以及尼泊尔的12个不同产区冬虫夏草样品和来自贵州省、江西省、湖南省的3种亚香棒虫草的δ15N和δ13C值。统计分析结果表明:不同来源的冬虫夏草样品之间δ13C值相互重叠,差异不明显,而δ15N值波动范围较大,除来源于青海海南州的1个样品外,其他冬虫夏草样品均可按地理来源进行有效区分;其中来源于青海玉树、果洛的优质冬虫夏草样品δ15N值与其他产地冬虫夏草样品存在显著差异(P<0.009);冬虫夏草样品与亚香棒虫草样品之间的δ15N、δ13C值存在显著差异。The isotopic composition of the elements N and C of twelve Ophicordyceps sinensis samples and three Cordyceps hawkesii samples was determined by isotope ratio mass spectrometry. The statistical analysis results showed that the δ13C value from different regions were overlap, but the δl5N value ranges were different and distinguished in almost all of the samples（except sample from Hainan of Qinghai）. The δ15N value from yushu and guoluo were signif- icantly different from other regions （P 〈 0. 009）. the δ15 N and δ13 C values between Ophicordyceps sinensis and Cordyceps hawkesii were significantly different. Therefore, Nitrogen stable isotope ratio analysis is useful in rapidly differentiating the geographical origin of Ophicordyceps sinensis. The method will have a wide application in tracing the origin of Ophicordyceps sinensis.

关 键 词：冬虫夏草 稳定同位素 产地鉴别 δ15N δ13C 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]