水稻新裂颖突变体sg1的遗传分析与基因定位  

Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice

作　　者：曾生元[1,2] 郭旻[1] 李荣德[1] 盛生兰[2] 龚红兵[2] 严长杰[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]江苏丘陵地区镇江农业科学研究所,江苏句容212400

出　　处：《中国水稻科学》2015年第1期22-27,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31171158);农业部转基因专项(2011ZX08009-003-005);江苏省自然科学基金资助项目(BK2012684;BK20141291);江苏省六大人才高峰项目;江苏高校优势学科建设工程项目;江苏省农业科技自主创新资金资助项目[CX(13)5073]

摘　　要：从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1,sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by 60 Co γ-ray. Compared to its wild type,the mutant has not only split glumes,but also characterized by early heading,lower plant height,reduced seed setting rate and degenerated spikelets,which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene,temporally designated as split glume 1 （sg1 ）,and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1 ,where six ORFs in total were predicted.On the basis of the location of SG1 ,it is deduced that SG1 is a novel gene regulating the rice glume development.

关 键 词：水稻 花器官 颖花开裂突变体 精细定位 sg1 

分 类 号：S511[农业科学—作物学]