云南哈尼梯田当前栽培水稻遗传多样性及群体结构分析  

Genetic Diversity and Population Structure Analysis of Currently Cultivated Rice Landraces from Hani's Terraced Fields in Yunnan Province

作　　者：刘承晨 赵富伟[3] 吴晓霞[2] 张昌泉 朱孔志 薛达元[3] 武建勇[3] 黄绍文[4] 徐小颖[2] 金银根[2] 刘巧泉[1,2] 

机构地区：[1]扬州大学江苏省作物遗传生理国家重点实验室培育建设点/粮食作物现代产业技术协同创新中心,江苏扬州225009 [2]扬州大学生物科学与技术学院,江苏扬州225009 [3]环境保护部南京环境科学研究所,南京210042 [4]云南红河学院,云南蒙自661100

出　　处：《中国水稻科学》2015年第1期28-34,共7页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2013CBA01402);国家自然科学基金资助项目(31300324);教育部重点项目;环境保护部生物多样性保护专项;江苏省高校"青蓝工程"资助项目;江苏省自然科学基金资助项目(13KJB180028)

摘　　要：利用118对 SSR 分子标记对2012年云南哈尼梯田的47份水稻材料进行多态性检测,分析其遗传多样性,并利用Structure 2．3．4软件进行群体结构分析.结果显示,118对 SSR 标记共检测到255个等位变异,变幅为2～4个,平均每个标记2．161个.基因多样性指数变异范围为0．043～0．656,平均0．303.多态信息含量（PIC）变异范围为0．042～0．583,平均0？256.其中高度多态位点（PIC＞0．5）有2个,中度多态位点（0．25＜PIC＜0．5）有66个；通过遗传相似系数及基于数学模型的群体结构分析均将供试材料分为偏籼和偏粳2个类群；聚类结果和海拔高度总体上没有明显规律,但偏籼类群中的偏籼红米亚群按海拔高低分别聚在一起.研究表明,云南哈尼梯田现有栽培水稻以偏籼水稻为主,但本研究估测的遗传多样性低于前人报道.In order to provide useful information for protection and utilization of rice landraces from Hani’s Terraced Fields,the genetic diversity and population structure of the currently cultivated lines were determined by using molecular makers.In this study,1 18 SSR markers were used to detect the polymorphism of 47 local rice materials,and their genetic diversity and population structure were analyzed.There were a total of 255 alleles,ranging from 2 to 4 per locus,with the mean of 2.1 6 1 .Genetic diversity index varied from 0.043 to 0.65 6 with an average of 0.303.The polymorphic information content （PIC）ranged from 0.042 to 0.583 with the average of 0.25 6.There are two highly polymorphic loci （PIC＆gt; 0.5 ）and 66 moderately polymorphic loci （0.25 ＆lt; PIC ＆lt; 0.5 ）.All the landraces could be divided into two groups,partial indica group and partial japonica group,based on the genetic similarity coefficient and mathematical model of population structure.There was no obvious regularity between clustering results and altitude, but partial indica red rice group had two clusters coming from different altitudes.In conclusion,currently cultivated rice in Hani’s Terraced Fields had a majority of landraces belonging to indica type,and lower genetic diversity than that in previous reports.

关 键 词：水稻 哈尼梯田 SSR 标记 遗传多样性 群体结构 

分 类 号：S511[农业科学—作物学]