维生素K2对肝癌细胞株内的肝癌衍生生长因子表达的抑制作用  

Inhibitory effect of vitamin K2 on expression of hepatoma-derived growth factor in hepatoma cells

作　　者：李鸣[1] 游建[1] 何鑫[1] 金鑫[1] 肖定[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院西院肝胆胰外科,武汉430034

出　　处：《中华实验外科杂志》2015年第1期67-68,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项目（2013CFB362）

摘　　要：目的 观察维生素K2对肝癌细胞株内的肝癌衍生生长因子（HDGF）表达的影响.方法 往培养板中加入不同浓度的维生素K2干预肝癌细胞培养,应用免疫组织化学方法检测肝癌细胞HDGF的表达水平.结果 HDGF阳性细胞细胞核和细胞质呈清晰的棕黄色颗粒,未干预组、15、30 μmol/L维生素K2干预组HDGF阳性表达率分别为79％、49％、23％.结论 维生素K2可明显抑制肝癌细胞中的肝癌衍生生长因子的表达,且呈现一定的剂量依赖性.Objective To study the effects of vitamin K2 on hepatoma-derived growth factor （HDGF） in a hapatoma cell line.Methods Different concentrations of vitamin K2 were added to the culture plates of hepatoma cells.The expression levels of HDGF in hepatoma cells were detected using immunohistochemistry.Results HDGF-positive cells in the nucleus and cytoplasm were shown as clear brown particles.The expression rate of HDGF was 79%,49% and 23% in the control group,the 15 μmol/L vitamin K2-treated group and the 30 μmol/L vitamin K2-treated group,respectively.Conclusion Vitamin K2 could significantly inhibit HDGF expression in hepatoma cells in a dose-dependent manner.

关 键 词：维生素K2 肝癌衍生生长因子 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]