大鼠肺缺血期肢体缺血处理对肺缺血/再灌注损伤的保护作用  

Protective effects of limbs ischemic preconditioning on lung ischemia-reperfusion injury in rats

作　　者：刘雷霆[1] 谭焱[2] 殷桂林[1,2] 朱水波[2] 胡赞歌 高旭辉[2] 张晓明[2] 

机构地区：[1]南方医科大学附属武汉临床学院,武汉430070 [2]广州军区武汉总医院心胸外科

出　　处：《中华实验外科杂志》2015年第1期88-90,共3页Chinese Journal of Experimental Surgery

基　　金：全军“十二五”医学科研资助项目（CWS11J019）

摘　　要：目的 观察大鼠肺缺血期双后肢缺血处理对肺缺血/再灌注损伤的影响.方法 将24只SD大鼠随机分为3组（n=8）：缺血再灌注组（I/R组）、肺缺血期肢体缺血处理组（LIPER组）和假手术组（S组）.I/R组开胸后左侧肺门阻断45 min,再灌注120 min.LIPER组左侧肺门阻断45 min,再灌注120 min,左侧肺门阻断5 min时阻断双后肢血供5 min,再灌注5 min,反复4次处理.S组开胸后旷置观察165 min.再灌注120 min时采动脉血样作血气分析;再灌注120 min后处死大鼠,取左肺组织,测定超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）活性及丙二醛（MDA）含量;计算湿干比（W/D）;肺组织行病理切片,苏木素-伊红（HE）染色,观察病理形态改变,并进行肺损伤评分.结果 I/R组和LIPER组血氧分压（PaO2）值分别为（58.5±3.1）和（71.0±3.5）mmHg（1 mmHg =0.133 kPa,P＜0.05）、W/D值分别为6.20±0.32和5.39 ±0.29（P ＜0.05）、SOD活性分别为（14.21±1.14）和（33.78 ±2.06） U/mg（P＜0.05）、MDA含量分别为（1.32±0.09）和（0.81±0.05） nmol/mg（P＜0.05）、MPO活性分别为（4.30 ±0.22）和（2.01 ±0.17） U/g（P＜0.05）、急性肺损伤评分值分别为（12.13±1.13）和（6.75±0.71）分（P＜0.05）.结论 肺缺血期双后肢缺血处理具有减轻肺缺血/再灌注损伤的作用.Objective To evaluate the effects of limbs ischemic preconditioning on lung ischemia-reperfusion injury in rats.Methods Twenty-four male healthy SD rats were randomly divided into 3 groups （n =8 each）：sham group （S group）,ischemia-reperfusion group （I/R group） and limbs ischemic preconditioning group （LIPER group）.In S group,animals underwent a sham thoracotomy and then the rats were observed for 165 min.In I/R group,the left hilum of lung was occluded with a noncrushing microvascular clamp for 45 min,followed by 120 min reperfusion.In LIPER group,the period of lung ischemia was held constant at 45 min,followed by 120 min reperfusion,and the rats underwent 4 episodes of 5 min legs ischemia at 5 min intervals after lung occlusion for 5 min.Arterial blood samples were taken for blood gas analysis at 120 min of reperfusion.Rats were killed at 120 min reperfusion and lungs were removed for determination of malondialdehyde （MDA） content,lung wet/dry weight ratio （W/D）,superoxide dismutase （SOD） and myeloperoxidase （MPO） activities.The lung tissues were subjected to histological examination under a light microscrope,and lung injury was sored.Results At the end of reperfusion,as compared with I/R group,acute lung injury scores in LIPER group were significantly reduced [（6.75 ± 0.71） vs.（12.13 ± 1.13）,P 〈0.05],pulmonary MDA content in LIPER group was reduced significantly [（0.81 ±0.05） vs.（1.32 ±0.09） nmol/mg,P〈0.05],MPO activity in LIPER group was decreased significantly [（2.01 ±0.17） vs.（4.30 ±0.22） U/g,P〈0.05],and arterial oxygen partial pressure in LIPER group was significantly increased [（71.0 ± 3.5） vs.（58.5 ± 3.1） mmHg （1 mmHg =0.133 kPa）,P 〈 0.05],and SOD activity in LIPER group was significantly increased [（33.78 ± 2.06）vs.（14.21 ±1.14） U/mg,P〈0.05].Conclusion Limbs ischemic preconditioning can attenuate the lung ischemia-reperfusion injury in rats.

关 键 词：肺损伤 肺缺血 再灌注损伤 肢体缺血预处理 

分 类 号：R655.3[医药卫生—外科学]