环孢素A对积水肾脏急性压力灌注损伤的保护  

The protective effect of cyclosporine A for acute renal function impairment induced by various of irrigation pressures in mild and severe hydronephrosis

作　　者：万鸿君[1] 姚小兵[1] 余伟民[1] 程帆[1] 李文[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《中华实验外科杂志》2015年第1期97-99,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 在不同程度肾脏积水的前提下,观察线粒体渗透性转换孔（MPTP）抑制剂环孢素A（CsA）对于急性压力灌注所致损伤的保护作用.方法 健康新西兰大白兔20只,随机分为轻度积水组（M组,n=10）和重度积水组（S组,n=10）,肾脏轻度积水及重度积水模型采用输尿管套扎法构建.M组及S组分为M1、M2、S1、S24个亚组,分别予以60、100、20、60 mmHg（1mmHg=0.133 kPa）压力灌注.灌注前M组及S组均给予CsA灌胃1周.灌注后48 h取标本,检测标本中超氧化物歧化酶（SOD）及丙二醛（MDA）的含量,JC-1染色检测线粒体膜电位（MMP）的变化.结果 M1、M2、S1、S2各组SOD活力分别为（30.11±2.23）、（28.41 ±2.26）、（44.82±6.61）、（40.31±5.21） U/mg,M1与M2之间SOD活力差异无统计学意义（P＞0.05）,S1与S2之间SOD活力差异无统计学意义（P＞0.05）;MDA的含量分别为（0.61 ±0.17）、（0.60±0.15）、（0.95 ±0.26）、（0.80±0.32） nmol/mg,M1与M2之间MDA含量差异无统计学意义（P＞0.05）,S1与S2之间MDA含量差异无统计学意义（P＞0.05）.JC-1染色橙红色阳性细胞百分比分别为（67.16±9.47）％、（60.75±11.89）％、（41.08 ±4.50）％、（42.36 ±3.44）％.M1与M2比较,S1与S2比较,阳性细胞百分比差异均无统计学意义（P＞0.05）.结论 CsA对于积水肾脏因急性压力灌注所致的损伤有一定的保护作用.在经皮肾镜及输尿管镜治疗结石前给予MPTP抑制剂可有效减轻压力灌注所致损伤.Objective To study the protective effect of cyclosporine A for acute renal function impairment induced by various of irrigation pressures in mild and severe hydronephrosis and to provided a reference values for clinical work during endourological procedures.Methods A total 20 rabbits were randomly divided into two groups：Mild hydronephrosis group （group M,n =10）,Severe hydronephrosis group （group S,n =10）.Both mild and severe hydronephrosis model were induced by embeding ureters into psoas.In each group,rabbits were randomly divided into 2 subgroups （M1,M2,S1 and S2） of respectively 5 rabbits.Groups M1,M2,S1 and S2 were respectively perfused with 60,100,20 and 100 mmHg （1 mmHg =0.133 kPa） pressure fluid.M and S groups were treated with CsA administered for one week before perfusion.The prefusion time was lasted for one hour.After that,the kidneys were harvested for measurement of super oxide dismutase （SOD） and malonaldehyde （MDA）.And the change of mitochondrial membrane potential was detected by JC-1 staining flow cytometry.Resets For group M1,M2,S1 and S2,SOD were （30.11 ±2.23）,（28.41 ±2.26）,（44.82 ±6.61）,（40.31 ±5.21） U/mg respectively; MDA were （0.61 ±0.17）,（0.60 ±0.15）,（0.95 ±0.26）,（0.80 ±0.32） nmol/mg respectively; the percentage of positive cell of expressing JC-1 were （67.16 ± 9.47）%,（60.75 ±11.89）%,（41.08 ±4.50）%,（42.36 ±3.44）% respectively.For M group,SOD,MDA and the percentage of positive cell has no significantly differences ; For S group,no abnormalities were observed.Conchsion CsA has a protective effect for damage due to renal perfusion.Give CsA before endosurgical procedures can effectively decrease damages induced by various of irrigation pressures.

关 键 词：肾积水 灌注压力 线粒体渗透性转换孔 损伤 

分 类 号：R692.2[医药卫生—泌尿科学]