β-受体激动剂质谱裂解特征研究  

Study on Mass Spectral Characteristics of β-receptor Agonists

作　　者：应永飞[1,2] 朱聪英[2] 周炜[2] 屈健[2] 陆春波[2] 汪以真[1] 

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]浙江省畜产品质量安全检测中心,杭州310020

出　　处：《中国兽药杂志》2015年第1期52-57,共6页Chinese Journal of Veterinary Drug

基　　金：公益性行业(农业)科研专项(201203023-4)

摘　　要：以克伦特罗、莱克多巴胺、沙丁胺醇为代表,阐明了β-受体激动剂在正离子软电离模式下的裂解机理。采用AB SCIEX 5500 QTRAP三重四级杆电喷雾质谱仪,运用IDA-EPI技术,对β-受体激动剂类化合物裂解规律进行研究解析。结果表明,在ESI正电离模式下β-受体激动剂容易获得H+而形成[M+H]+准分子离子峰,该结构易丢失水分子(H2O),继而与氨基结构相连的碳氮键(-C-N-)发生断裂,形成与苯环共轭的(Ph-CH=CH-NH+-)离子结构。本研究为同类化合物的结构解析提供了可靠依据以及跟踪新型β-受体激动剂提供了有力手段。To clarify pyrolysis mechanism of β- receptor agonists under positive mode, representative drugs including clenbuterol, salbutamol and ractopamine had been chosen to study with AB SCIEX 5500 QTRAP triple quadrupole mass spectrometry and IDA-EPI technology. The results showed that under ESI positive mode, β- receptor agonists were easy to form [ M＋H ] ＋. The structure was easy to loss neutral molecules, such as H20. Moreover, the C-N bond connected with amino group was easy to broken and produce conjugated ion structure with benzene. This study had important theory research significance and practical value, such as providing reliable basis for other β-receptor agonists structural analysis and providing powerful means to track new β-receptor agonists.

关 键 词：β-受体激动剂 质谱裂解 液相色谱-串联质谱法 

分 类 号：S859.83[农业科学—临床兽医学]