广州市某绿色和有机蔬菜基地土壤中四环素类抗生素的含量与分布特征  

Concentrations and Distributions of Tetracycline Antibiotics in Soils of Green and Organic Vegetable Fields in Guangzhou, China

作　　者：邰义萍[1,2,3] 莫测辉[3] 李彦文[3] 吴小莲[3] 黄献培[3] 向垒[3] 

机构地区：[1]中国科学院华南植物园,广州510650 [2]中国科学院大学,北京100049 [3]暨南大学环境工程系广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州510632

出　　处：《农业环境科学学报》2014年第9期1743-1748,共6页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(41173101;41301337);广东省自然科学基金重点项目(2011020003196);中央高校基本科研业务费专项资金项目(21612103);广东省科技计划项目(2010B020311006);广东省高校高层次人才项目;广州市科技计划项目(2010A82070466);东莞市科技研究计划项目(201210815000399)

摘　　要：利用高效液相色谱-串联质谱（HPLC-MS/MS）分析方法,探讨了广州地区施用粪肥的＂绿色蔬菜基地＂和＂有机蔬菜基地＂土壤中4种四环素类抗生素（四环素、土霉素、金霉素和强力霉素）的含量与分布特征。结果表明：土壤中4种四环素类抗生素的总含量在0.11～48.45μg·kg^-1之间,平均为12.64μg·kg^-1;各抗生素的检出率为69%～92%,平均含量为0.98～6.59μg·kg^-1,以土霉素为主;有机蔬菜基地土壤中四环素类抗生素的平均总含量比绿色蔬菜基地土壤中的高;同一基地不同品种蔬菜地土壤中4种四环素类抗生素的组成及含量特征有一定差异。研究区土壤中四环素类抗生素的含量低于土壤中抗生素生态毒害效应触发值（100μg·kg^-1）,对土壤生物群落结构与功能的生态毒害风险较小。Extensive uses of tetracycline antibiotics（TCs）in animal production have caused soil contamination via manure applications. In this study, we investigated the concentrations and distributions of four TCs including tetracycline, oxytetracycline, chlortetracycline and deoxytetracycline in soils from green and organic vegetable fields in Guangzhou using high performance liquid chromatography-electrospray ionization tandem mass spectrometry（HPLC-MS/MS）. Total concentrations of these four TCs ranged from 0.11 μg·kg^-1 to 48.45 μg·kg^-1,averaging at 12.64 μg·kg^-1. Detection frequencies were 69% to 92% for the soil samples. The average concentration of individual antibiotics was from 0.98 μg·kg^-1to 6.59 μg·kg^-1, dominanet with oxytetracycline. Higher TCs concentrations were found in organic than in green vegetable fields. Concentrations and composition of tetracycline compounds in soils were different among different varieties or genotype of vegetables. So far the ecological risk of these four TCs in the soils was low as their concentrations were well below the ecotoxic effect trigger value（100 μg·kg^-1）set by the Steering Committee of Veterinary International Committee on Harmonization. Further investigation including environmental fate, plant uptake, and human exposure to antibiotics via plant-derived food should be conducted.

关 键 词：绿色蔬菜基地 有机蔬菜基地 四环素类抗生素 土壤污染 

分 类 号：X592[环境科学与工程—环境工程]