AMF对镉污染条件下水稻抗逆性及根际固定性的影响  

Effects of Arbuscular Mycorrhizal Fungi on Cadmium Tolerance and Rhizospheric Fixation of Rice

作　　者：王立[1] 安广楠 马放[1] 吴洁婷[1] 张雪[1] 王敏[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《农业环境科学学报》2014年第10期1882-1889,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金(51179041);水体污染控制与治理科技重大专项(2012ZX07201003);城市水资源与水环境国家重点实验室自主课题(2014TS05)

摘　　要：为研究丛枝菌根真菌对重金属Cd胁迫条件下水稻生长及生理过程的影响,选用两种丛枝菌根真菌(Arbuscularmy corrhizal fungi,AMF):摩西球囊霉(Glomus mosseae,GM)和根内球囊霉(Glomus intraradice,GI),接种在不同Cd浓度(0、0.5、1、2、5 mg·kg-1)的污染土壤中,研究两种菌剂对水稻株高、光合作用、抗逆生理过程以及Cd在植株地上及根系分配的影响。结果表明,AMF菌剂的接种显著减轻了Cd胁迫对水稻生长的抑制程度,GM组与GI组水稻株高分别比对照组提高了13.8%和10.3%,且菌剂处理有助于缓解Cd胁迫对水稻光合作用的抑制。在5 mg·kg-1Cd胁迫下,水稻叶片中MDA(丙二醛)含量显著降低,SOD(超氧化物歧化酶)活性和Pro(脯氨酸)的含量显著提高(P<0.05)。两种菌剂处理下水稻对Cd的富集系数与转运系数均低于对照,表明AMF处理有助于缓解Cd对地上部分的毒害,并且促进了水稻对Cd的根际固定化过程,抑制了Cd向地上部分转移的趋势。Arbuscular mycorrhizal fungi（AMF）have showed to improve plant resistance to and reduce translocation of heavy metals. Here we investigated the effects of Glomus mosseae（GM）and Glomus intraradices（GI）on the growth and physiological processes of paddy rice （Oryza sativa L.）grown in soil spiked with 0, 0.5, 1.0, 2.0 and 5.0 mg Cd·kg-1. Mycorrhizal inoculation significantly alleviated the inhibi-tion of rice growth caused by Cd stress. Plant height increased by 13.8%（GM）and 10.3%（GI）compared to the control; Inoculating AMF also relieved the inhibitory effect of Cd on rice photosynthesis. At the highest concentration of Cd in this experiment（5.0 mg·kg-1）, the con-tent of malondialdehyde（MDA）decreased significantly while superoxide dismutase（SOD）activity and proline（Pro）concentration in-creased remarkbly（P〈0.05）. Compared with the control, AMF inoculation promoted the Cd immobilization in roots and prevented the Cd transfer from roots to aboveground parts. Bioconcentration factor（BCF）and translocation factor（TF）were both lower in GM and GI treatments than those in the control, implying that AMF could reduce Cd influence on aboveground part of paddy rice.

关 键 词：摩西球囊霉 根内球囊霉 水稻 镉胁迫 根际固定化 

分 类 号：X503.231[环境科学与工程—环境工程]