维药刺山柑抑制金黄色葡萄球菌生物膜的研究  

Effect the biofilm formation of Staphylococcus aureus by Xinjiang Capparis spinosa

作　　者：徐琦[1] 吴晔华[1] 王晓梅[2] 李雪亮[1] 谢天宇[1] 马梦佳[1] 杨学安[1] 薛志琴[1] 

机构地区：[1]新疆医科大学基础医学院,新疆乌鲁木齐830011 [2]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《中国医药导报》2015年第2期20-23,29,共5页China Medical Herald

基　　金：中国科学院重点实验室-干旱区植物资源化学实验室开放课题(编号2008DP173091-2012-01)

摘　　要：目的研究维药刺山柑提取及萃取物对金黄色葡萄球菌体外培养生物膜(BF)形成及清除作用的影响,为进一步开发维药刺山柑的应用价值及临床辅助治疗耐药性感染提供实验依据。方法通过醇提法获得维药刺山柑醇提物,并通过石油醚、乙酸乙酯及正丁醇的萃取获得不同极性的萃取物;使用96孔板培养金黄色葡萄球菌体外BF模型,采用试管二倍稀释法测定维药刺山柑不同提取物/萃取物的最低抑菌浓度(MIC),研究其对金黄色葡萄球菌BF形成的影响;同时通过检测维药刺山柑提取物对成熟BF中活菌数的影响,评价其对成熟BF的清除作用。结果维药刺山柑醇提物、石油醚萃取物、乙酸乙酯萃取物以及正丁醇萃取物对金黄色葡萄球菌的MIC分别为52.50、50.00、45.00、46.25 mg/m L。当浓度分别为26.26、23.13、25.00、22.50 mg/m L时维药刺山柑醇提物及不同萃取物可在早期阶段干扰金黄色葡萄球菌BF的形成,致活菌数明显少于空白对照组,差异有统计学意义(P<0.05),其中4倍MIC浓度的正丁醇萃取物抗菌活性明显,与青霉素组比较,差异无统计学意义(P>0.05),其中高浓度醇提物及正丁醇萃取物24 h清除BF的作用较强,与空白对照组比较,差异有统计学意义(P<0.05);维药刺山柑醇提物及不同萃取物还可以抑制并有效清除金葡菌成熟BF,其高浓度抗菌活性与青霉素比较,差异无统计学意义(P>0.05)。结论维药刺山柑醇提物及不同溶剂萃取物在体外具有抑制金黄色葡萄球菌BF形成的作用,并且能通过破坏已形成的BF起到杀灭细菌的作用,为临床辅助治疗耐药性金葡菌感染,提供实验依据。Objective To study the effect of Capparis spinosa. extract on Staphylococcus aureus’biofilm （BF） in vitro, and provide the experimental basis of clinical application. Methods The Capparis spinosa. extract by alcohol extraction was obtained, and the different polarity extracts was obtained by extraction with petroleum ether, ethyl acetate and n-butanol; the Staphylococcus aureus BF in v itro was induced by 96-well plates, the determination of the tube double dilution method was used to measure the minimal inhibitory concentrations （MICs） in different extracts/extraction from Cap-paris spinosa., the impact of BF formation for Staphylococcus aureus was evaluated. At the same time, the evaluation of its scavenging effect on mature BF was done by detecting the impact of Uyghur medicine Capparis spinosa. extract on the number of living bacterium in mature BF. Results The MIC for Staphylococcus aureus in Capparis spinosa. alcohol extract, petroleum ether extraction, ethyl acetate extracts were 52.50, 50.00, 45.00, 46.25 mg/mL, respectively. When the concentration reached 26.26, 23.13, 25.00, 22.50 mg/mL, alcohol extraction, ethyl acetate and n-butanol samples could inhibit the formation of BF, the number of living bacterium was significantly less than the blank control group, the difference was statistically significant （P〈 0.05）. Among them, four times the concentration of MIC n-butyl alcohol extract antibacterial activity was significantly obvious, compared with Penicillin group, there was no statistically significant difference （P〉0.05）, the role of clearing BF of the high concentration of alcohol extract and n-bu-tanol extract was stronger in 24 h, compared with the blank control group, there was significant difference （P〈0.05）; Uyghur medicine Capparis spinosa. alcohol extract＆amp;nbsp;and different extract could inhibit and effectively remove Staphylococcus aureus mature BF, compared with Penicillin, there was no statistically significant difference in its high antibacterial activity

关 键 词：金黄色葡萄球菌 维药刺山柑 生物膜 

分 类 号：R258.5[医药卫生—中医内科学]