Development and Characterization of Fifteen Polymorphic M icosatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis  

作　　者：Weizhao YANG Jie WANG Jianping JIANG Cheng LI Feng XIE 

机构地区：[1]Chengdu Institute of Biology, Chinese Academy of Sciences

出　　处：《Asian Herpetological Research》2014年第4期281-283,共3页亚洲两栖爬行动物研究（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (30770316)

摘　　要：Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. ehinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. ehinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.

关 键 词：Echinotriton chinhaiensis polymorphic microsatellite loci population genetics conservation biology 

分 类 号：Q953[生物学—动物学]