基于主成分分析模式对不同产地梅花鹿茸HPLC指纹图谱研究  

HPLC fingerprint of the young horn of Cervus Nippon Temminck from different habitats based on principal component analysis

作　　者：曹越[1] 田淋淋 李航[2] 张美双[2] 宗颖[2] 刘冬[2] 张辉[2] 孙佳明[2] 

机构地区：[1]长春中医药大学药学院,长春130117 [2]长春中医药大学中医药与生物工程研发中心,长春130117

出　　处：《吉林中医药》2014年第12期1258-1260,共3页Jilin Journal of Chinese Medicine

基　　金：国家自然基金课题(81373936);吉林省科技发展计划项目(20140203015YY);吉林省卫生厅科研课题(2013Z041);吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2014]第73号)

摘　　要：目的建立不同产地梅花鹿茸的HPLC指纹图谱,并对梅花鹿茸和马鹿茸的指纹图谱进行模式识别研究。方法利用HPLC-DAD方法,以不同产地梅花鹿茸药材为研究对象,对10批药材进行了指纹图谱分析,同时运用主成分分析对梅花鹿茸和马鹿茸进行模式识别研究。结果建立了10批梅花鹿茸药材的指纹图谱,确定了10个共有峰,通过主成分分析将梅花鹿茸和马鹿茸分为3类。结论所建立的HPLC指纹图谱及主成分分析模式方法具有快速稳定的特点。Objective To establish the HPLC fingerprint analysis of the young horn of Cervus Nippon Temminck collected from different habitats. And study pattem recognition of the young horn of Cervus Nippon Temminck（ MHLR）and Cervus elaphus Linnaeus（ MLR）. Methods 10 batches of the young horn of Cervus Nippon Temminck come from the different habitats were carried out to gain fingerprints by HPLC-DAD. To study pattem recognition of the young horn of Cervus Nippon Temminck and Cervus elaphus Linnaeus by principal component analysis（ PCA）. Results 10 common peaks were identified from the 10 batches of the samples. The young horn of Cervus Nippon Temminck and Cervus elaphus Linnaeus were classified into 3 categories by PCA. Conclusion The method of the HPLC fingerprint established by PCA can be used for the classification of the young horn of Cervus Nippon Temminck and has the characteristic of rapidness and stability.

关 键 词：梅花鹿茸 HPLC指纹图谱 主成分分析 

分 类 号：R282.74[医药卫生—中药学]