不同时机给药对原发性痛经模型小鼠雌激素受体、缩宫素受体的影响  

Effect of different administration time on ER-α and OTR of mice with primary dysmenorrheal

作　　者：蒲宝婵[1] 李爱主 方玲[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《吉林中医药》2014年第12期1270-1272,1276,共4页Jilin Journal of Chinese Medicine

基　　金：天津市应用基础与前沿技术研究计划项目(13JCYBJC23900)

摘　　要：目的观察不同时机给药对原发性痛经模型小鼠雌激素受体(ER-α)和缩宫素受体(OTR)的影响。方法将km小鼠按体重分层随机分为:JQF动情周期给药组、JQF第4天给药组(JQF剂量均为0.218 g/kg)、元胡止痛片组(0.1 g/kg)、模型组和正常组。除正常组外每日皮下注射苯甲酸雌二醇0.2 mg/只,连续6 d。采用Western blot和Real-time PCR分别检测1周期给药和3周期连续给药对ER-α和OTR蛋白与m RNA表达的影响。结果与模型组比较,JQF动情周期给药组与第4天给药组ER-α和OTR蛋白、m RNA的表达均呈现显著下调(P<0.01)。动情周期给药组和第4天给药组组间比较无差异,3周期连续给药实验结果与1周期一致。结论不同时机给药对原发性痛经模型小鼠ER-α和OTR蛋白、m RNA表达有相同的抑制作用。Objective To study the effect of different administration time on ER-α and OTR of mice with primary dysmenorrheal. Methods The km mice were randomly divided into the JQF estrus cycle group,the JQF 4th day group（ JQF dosage,0. 218 g /kg accordingly）,the Yuanhu Zhitong Tablets group（ 0. 1 g /kg）,model group and normal control group. The mice received daily subcutaneous injections of estradiol benzoate 0. 2 mg per one for 6 consecutive days except the normal control group when in the proestrus. The effect of a cycle of administration and the other three cycles with continuous administration expressions on OTR and ER-α was detected by Western blot and Real-time PCR. Results The expressions of ER-α,OTR protein and m RNA were significantly reduced in the estrus cycle group and the4 th day group（ P〈0. 01）,compared with the model control group. The comparison between the estrus cycle group and the 4th day group had no difference. The results of three cycles were consistent with one cycle. Conclusion Different administration time on ER-α,OTR and m RNA of mice with primary dysmenorrheal has the same inhibitory action.

关 键 词：原发性痛经 不同时机给药 ER-Α OTR 动情周期 小鼠 

分 类 号：R285.5[医药卫生—中药学]