还原型谷胱甘肽与多烯磷脂酰胆碱对环磷酰胺诱导小鼠肝损伤的修复作用  

Repairing effects of reduced glutathione and polyene phosphatidyl choline on liver injury evoked by cyclophosphamide in mice

作　　者：孙向菊[1] 吴玉波[1] 蒋爱华[1] 徐娜[1] 梁晶[1] 吴禹蒙[1] 

机构地区：[1]哈尔滨医科大学附属第四医院药剂科,150010

出　　处：《药物不良反应杂志》2014年第6期345-349,共5页Adverse Drug Reactions Journal

摘　　要：目的：分析比较还原型谷胱甘肽与多烯磷脂酰胆碱对环磷酰胺诱导小鼠肝损伤的防护作用。方法采用简单随机抽样法将40只昆明小鼠分为肝损伤模型组、还原型谷胱甘肽组、多烯磷脂酰胆碱组和对照组，每组10只。前3组小鼠实验第1-4天均腹腔注射环磷酰胺（100 mg· kg-1·d-1）诱导肝损伤，第5-14天分别腹腔注射0.9%氯化钠注射液0.2 ml、还原型谷胱甘肽180 mg · kg-1· d-1、多烯磷脂酰胆碱90 mg·kg-1·d-1；对照组同期腹腔注射等体积0.9%氯化钠注射液。实验第1天给药前和第15天测定小鼠体重；实验第15天，小鼠处死前眼眶取血测定血清总胆红素和谷胱甘肽水平，处死小鼠后取肝脏称重并计算肝脏系数，取肝组织测定丙氨酸转氨酶（ ALT）、天冬氨酸转氨酶（AST）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和丙二醛（MDA）水平，并进行肝组织形态学观察。结果实验第15天，应用环磷酰胺的3组小鼠体重均明显低于对照组（P 〈0.01或 P 〈0.05），但还原型谷胱甘肽组体重高于肝损伤模型组（P 〈0.05）；肝损伤模型组小鼠肝脏系数（5.74%±0.11%）高于对照组（4.68%±0.37%）和还原型谷胱甘肽组（4.81%±0.19%）（均 P 〈0.01），多烯磷脂酰胆碱组小鼠肝脏系数（5.25%±0.35%）]也高于对照组（P 〈0.05）。肝损伤模型组、还原型谷胱甘肽组、多烯磷脂酰胆碱组血清总胆红素水平[（129.8±1.9）、（110.9±1.3）、（125.7±2.6）μmol/ L]均高于对照组（100.8±3.0）μmol/ L（均 P 〈0.01），但还原型谷胱甘肽组低于肝损伤模型组（P 〈0.01）。肝损伤模型组和多烯磷脂酰胆碱组血清谷胱甘肽水平[（50.5±1.9）、（55.9±2.4）g/ L]均低于对照组和还原型谷胱甘肽组[（73.8±4.3）、（71.3±3.7）g/ L]（均 P 〈0.01）。肝损伤模型组、还原型谷胱甘肽组、多烯磷脂酰胆碱组肝组织 AST、ALT、SOD 和 CAT �Objective To analyze and compare the prothetic effects of reduced glutathione and polyene phosphatidyl choline on liver injury evoked by cyclophosphamide in mice. Methods Forty Kunming mice were divided into the liver injury model group,the reduced glutathione group,the polyene phosphatidyl choline group,and the control group by simple random sampling. Each group comprised 10 mice. The mice in the fornamed 3 groups received intraperitoneal injection of cyclophosphamide（100 mg ·kg-1 ·d-1 ）to evoke the liver injury on day 1 to 4 of the experiment and 0. 9% sodium chloride solution 0. 2 ml,reduced glutathione 180 mg· kg-1 · d-1 ,and polyene phosphatidyl choline 90 mg·kg-1 ·d-1 were given respectively in the 3 groups on day 5 to 14 of the experiment. The mice in the control group received intraperitoneal injection of same volume of 0. 9% sodium chloride solution on day 1 to 14 of experiment. The body weight of mice in the 4 groups were measured on the first day before intraperitoneal injection of cyclophosphamide and the fifteenth day of the experiment. The mice in the 4 groups were executed on the fifteenth day of the experiment. The blood samples were taken from eye pit before the mice were killed and the serum total bilirubin and glutathione levels were measured. Their liver tissue were taken and weighed, and the liver coefficient were calculated. The liver tissue were taken and the activities of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,superoxide dismutase（ SOD）,catalase （CAT）and the level of malonaldehyde（ MDA）in the liver tissue were measured. The morphological changes in the liver tissue in the 4 groups were observed. Results On day 15 of experi-ment,the body weights in mice in the liver injury model group,the reduced glutathione group,and the polyene phosphatidyl choline group were lower significantly than those in the control group（P 〈 0. 01 or P 〈 0. 05）. The body weights in mice in the reduced glutathione group were higher than those in the liver

关 键 词：环磷酰胺 谷胱甘肽 磷脂酰胆碱类 药物性肝损伤 

分 类 号：R965[医药卫生—药理学]