正相高效液相色谱法同时测定化妆品中2种熊果苷含量  

Simultaneous determination of α-,β-arbutin in cosmetics by normal phase high performance liquid chromatography

作　　者：张乐[1] 王卫华[2] 姜艳艳[1] 杨元[1] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国计量科学研究院,北京100013

出　　处：《日用化学工业》2015年第1期55-57,59,共4页China Surfactant Detergent & Cosmetics

基　　金：国家质检总局公益行业专项资助项目(2012104001);北京中医药大学创新团队资助项目(2011-CXTD-12)

摘　　要：采用正相高效液相色谱,建立了化妆品中α-,β-熊果苷的含量测定方法。采用S-Chiral A色谱柱（250 mm×4.6 mm,5μm）和二极管阵列检测器,以V（正己烷）：V（乙醇）=70：30的混合溶液为流动相,流速为0.8 m L/min,检测波长为226 nm,柱温为30℃。α-,β-熊果苷的检出限（S/N=3）分别为1.5和3.6 ng,定量限（S/N=10）分别为1.6和6.3 ng,10～500 mg/L内,峰面积与质量浓度呈良好的线性关系,相关系数分别为1.000 0和0.999 0。高、中、低不同水平的加标回收率为99.61%～103.36%,RSD不大于0.80%（n=6）。该方法操作简便,准确度、灵敏度和稳定性高,可用于化妆品中α-,β-熊果苷的含量测定。An analysis method was established for determination of α - ,β- arbutin in cosmetics by normal phase high performance liquid chromatography. The separation was performed on an S- Chiral A column （250 mm ×4.6 mm,5μm） with n -hexane -ethanol （volume ratio 70： 30） as the mobile phase at a flow rate of 0. 8 mL/min. The detection wavelength was 226 nm. Operating temperature was 30 ℃. The limit of detection of α - ,β - arbutin are 1.5 and 3.6 ng respectively, and the limit of quantification are 1.6 and 6. 3 ng respectively. A good linear relationship is obtained between the peak area and the mass concentration of α - ,β - arbutin in the range of 10 - 500 mg/L and the correlation coefficient are 1. 000 0 and 0. 999 0 respectively. The recoveries of α - ,β - arbutin at different spiked level are 99.61% - 103.36% with the relative standard deviations （RSD） no more than 0. 80%.

关 键 词：化妆品 正相高效液相色谱法 α-熊果苷 β-熊果苷 测定 

分 类 号：TQ658[化学工程—精细化工] O657.72[理学—分析化学]