泽丹冲剂对盆腔炎性大鼠子宫TGF-β_1、Smad_3的影响研究  

Effects of Zedan infusion on Smad3 and TGF-β1 in rats with chronic pelvic inflammatory disease

作　　者：谷风[1] 陶红星[2] 沈祖泓[1] 曾远强 罗彩萍[1] 张敏玲[1] 苗久旺[3] 

机构地区：[1]广州中医药大学附属清远中医院,清远511500 [2]深圳市南山区人民医院,深圳518000 [3]山东中医药高等专科学校,烟台265200

出　　处：《陕西中医》2015年第1期120-122,共3页Shaanxi Journal of Traditional Chinese Medicine

基　　金：广东省清远市科技局博士项目(2013A012)

摘　　要：目的:研究泽丹冲剂对慢性盆腔炎大鼠子宫组织TGF-β1、Smad3表达的影响,阐明以利湿化瘀法为主的泽丹冲剂作用机制。方法:大鼠双侧宫腔内注射大肠杆菌混悬液(浓度约为6×106)0.2mL建立慢性盆腔炎大鼠模型。造模成功后,随机分成泽丹冲剂高、中(8g·kg、4g·kg)剂量组,妇乐颗粒组(2.5g·kg),模型组和正常组。灌胃给药21d后,采用Western Blot法检测大鼠子宫组织TGF-β1、Smad3表达。结果:与模型组比较,泽中、高剂量组,妇乐组子宫组织TGF-β1、Smad3表达均明显降低(P<0.05),但泽中、高剂量组优于妇乐组(P<0.05)。结论:泽丹冲剂通过降低TGF-β1、Smad3的异常高表达,减轻慢性盆腔炎大鼠子宫组织的纤维化及粘连,因而对慢性盆腔炎具有良好的治疗作用。Objective：To observe the effects of Zedan Infusion on Smad3 and TGF‐β1 in the rats with Chronic pelvic inflammatory disease ,so as to discuss its mechanism .Methods ：The rat model of chronic pelvic inflammatory dis‐ease was established by injecting coli suspension （concentration of about 6 × 106 ） 0 .2ml to the bilateral intrauterine .After the modeling ,the rats were randomly divided into Zedan Infusion high and medium （8g·kg ,4g·kg） dose group ,Fule particle group （2 .5g·kg） ,model group and the normal group .After oral administration 21d ,the expression of TGF‐β1 and Smad3 was assayed by the Western Blot .Results ：Compared with the model group ,the expression of TGF‐β1 and Smad3 was significantly lower （P〈0 .05） ,which was better in Zedan infusion high and medium dose group than in Fule group （P〈0 .05） .Conclusion：Zedan infusion can reduce the abnormal expressions of TGF-β1 and Smad3 ,has anti-tis‐sue adhesions and fibrosis effects ,thus it has good clinical efficacy on Chronic pelvic inflammatory disease .

关 键 词：盆腔炎性疾病/中医药疗法 动物实验 大鼠 @泽丹冲剂 

分 类 号：R711.33[医药卫生—妇产科学]