鼠尾藻微卫星标记开发及青岛群体遗传多样性分析  

Development of Sargassum thunbergii Microsatellites and Genetic Diversity Description of Qingdao Population

作　　者：汤志宏[1] 刘振羽[1] 王军[1] 张宁[1] 毛玉峰[1] 郭栗[1] 杨官品[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2015年第1期35-40,共6页Periodical of Ocean University of China

基　　金：国家海洋局公益项目(201205024-02)资助

摘　　要：分离了鼠尾藻14个微卫星标记并用这些标记分析了鼠尾藻青岛群体遗传多样性和遗传结构。开发的14个标记在青岛群体中共检测到45个等位基因,各标记位点等位基因数在2和6之间,平均3.2个,平均有效等位基因2.1个,群体香农信息指数、观测杂合度、基因多样性指数、多态信息含量分别为0.799、0.449、0.466和0.406。青岛鼠尾藻种群体遗传多态性中等偏高。青岛4个鼠尾藻采集点的56个鼠尾藻个体在聚类图上无明显聚群和谱系。开发的标记将促进鼠尾藻遗传资源评价、保护和开发利用。Fourteen microsatellites of Sargassum thunbergii were isolated and used to determining the genetic diversity of Qingdao population.These microsatellites detected 45 alleles in total in Qingdao population and the number of alleles varied between 2 and 6,3.2 in average.The number of average effective alleles of Qingdao population was 2.1,and the mean value of Shannon＇s information index,observed heterozygosity,gene diversity and polymorphism information content was 0.799,0.449,0.466 and 0.406,respectively.The genetic polymorphism of Qingdao S.thunbergii was at the medium level.The 56 Sthunbergii individuals of Qingdao population scattered each other.These microsatellites will facilitate the evaluation,protection and exploitation of S.thunbergii genetic resource.

关 键 词：鼠尾藻 微卫星标记 遗传多样性 

分 类 号：S917.3[农业科学—水产科学] Q322[生物学—遗传学]