新疆陆地棉转天麻毒蛋白基因GAFP特性研究  

Study on the Characteristics of Transgenic(GAFP)Xinjiang Upland Cotton

作　　者：沈海涛[1,2] 王爱英[1,2] 李玉霞[1] 祝建波[1,2] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学,农业生物技术重点实验室,新疆石河子832003

出　　处：《云南农业大学学报（自然科学版）》2015年第1期1-8,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家转基因专项(2011zx08011-002);新疆生产建设兵团青年科技创新资金专项(2013cb010)

摘　　要：本研究利用花粉管通道技术将在天麻中克隆的天麻毒蛋白基因(GAFP)转化棉花,经PCR和RTPCR鉴定,证实得到了5个转基因株系672GAFP-1,672GAFP-2,672GAFP-3,672GAFP-4,672GAFP-5。通过两年在无病田和病圃中连续种植分析转基因植株抗病性、农艺性状和经济性状的研究,结果表明:外源基因GAFP的导入对棉花农艺性状和经济性状无显著影响;转基因株系672GAFP-1,672GAFP-2,672GAFP-5在病圃中的抗病指数、株高、产量和纤维品质与受体材料672差异显著(P<0.05),产量较对照提高近30%;转基因植株农艺性状和经济性状的各项指标均优于对照,具较强耐枯、黄萎病能力,且能稳定遗传。In this study, the gene of Gastrodia antifungal protein （GAFP） was cloned and transferred into one cuhivar of land cotton using the method of pollen-tube-channel, with the purpose of obtaining transgenic plants with improved resistance to wilt. Five transgenic cottons, including 672GAFP-1, 672GAFP-2, 672GAFP-3, 672GAFP-4 and 672GAFP-5, were obtained and proved based on RCR and RT-PCR. After breeding for two years, the progeny of 672GAFP-1, 672GAFP-2 and 672GAFP-5 lines showed a stable and strong resistance to Verticillium wilt and Fusarium wilt. Field analysis showed that the transgenie cotton yield increased by 30% more than that of non-transgenie, agronomic characters and fiber quality analysis showed that the transgenie cotton was better than non-transgenic in disease nursery, but they were not different in field of free disease. Lines with high levels of resistance to Verticillium wilt and Fusarium wilt obtained from the present study may be widely planted and help to reduce the future impact of cotton wilt on production resulting in increased yields.

关 键 词：天麻抗真菌蛋白 转基因抗病陆地棉 棉花枯萎病 黄萎病 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]