柑橘褐斑病菌菌丝生长和孢子萌发研究  

Study on Relation of Mycelial Growth and Spore Germination of Alternaria alternate Caused Occurrence of Citrus Brown Spot

作　　者：胡中会[1] 赵立华[2] 张铁[1] 

机构地区：[1]文山学院资源与环境学院,云南文山663000 [2]云南农业大学植物保护学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2015年第1期9-12,共4页Journal of Yunnan Agricultural University:Natural Science

摘　　要：对柑橘褐斑病病原菌链格孢菌（Alternaria alternata）生物学特性的研究结果表明：5种供试碳源都能被利用,但菌丝生长和孢子萌发情况存在差别,以甘露醇为碳源时菌丝生长和孢子萌发最好,以蔗糖为碳源时菌丝生长最差,以麦芽糖为碳源时孢子萌发最差。5种供试氮源都能被利用,但菌丝生长和孢子萌发情况存在差别,以甘氨酸为氮源时菌丝生长和孢子萌发最好,硝酸铵菌丝生长最差,硫酸铵孢子萌发最差。温度、pH值对菌丝生长和孢子萌发有一定影响,最适生长和孢子萌发温度为21℃,最适p H值为5～8。光照对其生长和孢子萌发影响不明显。Biological characteristics of Alternaria alternate, which caused occurrence of Citrus Brown Spot, were studied. The results indicated that five carbon sources could be used, but the conditions between mycelium growth and spore germination were different. The most suitable carbon source for mycelium growth and spore germination was mannitol, and sucrose was not suitable carbon source for mycelium growth and maltose was not suitable for spore germination. Although all of five nitrogen sources could be utilized by strain growth, the conditions between mycelium growth and spore germination were various. The most suitable nitrogen source for mycelium growth and spore germination was glycin. In the nitrogen sources trial, it revealed that ammonium nitrate was the worst for mycelium growth and ammonium sulfate was the worst for spore germination. Temperature and pH had effects on the mycelium growth and spore germination, the suitable temperature for mycelium growth and spore germination was 21℃, it could grow well under the pH value of 5 -8. The results also indicated that the treatment with different light conditions did not significantly affect the mycelium growth and spore germination.

关 键 词：柑橘褐斑病 链格孢菌 菌丝生长 孢子萌发 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治]