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作 者:高杨[1] 高巍[1] 陈腾[1] 张梅[1] 倪传武 刘晨黎[1] 陈道富[1]
出 处:《动物营养学报》2015年第1期274-280,共7页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:国家自然科学基金项目(31260557);兵团博士资金专项(2013BB018)
摘 要:本试验旨在研究哈萨克羊育肥羊在以低质粗饲料条件下,增加饲粮瘤胃降解蛋白质(RDP)水平对瘤胃微生物氮(MN)合成量和氮沉积量的影响。选取体重为(30.0±2.8)kg的哈萨克羊育肥羊公羔3只作为试验动物,分别饲喂RDP水平为7.94%(TMR1)、9.02%(TMR2)、10.10%(TM R3)的3种饲粮。按照3×3拉丁方试验设计进行试验,共3期,每期预试期10 d,正试期5 d。全部收集粪尿,测定尿中嘌呤衍生物(PD)排出量、瘤胃MN合成量和转化效率。结果表明:3种饲粮的干物质及有机物的采食量及表观消化率均无显著性差异(P>0.05),但TM R3的氮沉积量、尿中PD排出量及瘤胃M N的合成量均显著高于TM R1和TM R2(P<0.05)。饲粮RDP水平与MN合成量/瘤胃表观可消化有机物(RADOM)采食量之间存在显著的正相关(R2=0.999 4,P=0.010 9)。M CP的转化效率不受饲粮RDP水平影响(P>0.05)。结果提示,低质粗饲料条件下,将饲粮RDP水平由7.94%提高至10.10%,能够提高哈萨克育肥羊氮沉积量,促进瘤胃MCP的合成,但未能显著提高MCP的转化效率。The aim of this study was to investigate the effects of increased dietary rumen degradable protein ( RDP) level on rumen microbial nitrogen ( MN) yield and nitrogen retention of finishing Kazakh sheep under inferior forage feeding condition. Three lambs weighed (30.0±2.8) kg were fed three diets with different RDP levels [7.94%(TMR1), 9.02%(TMR2) and 10.10% (TMR3)]. A 3&#215;3 Latin square experimental design was adopted. There were three periods with 10-day pretest period and 5-day test period in each period. Total collection of feces and urine method was used to determine urinary purine derivatives ( PD) excretion, rumen MN yield and conversion rate. The results showed as follows:the intake and apparent digestibility of dry matter ( DM) and organic matter ( OM) of the three diets were not significantly different ( P〉0.05) , however, nitrogen retention, urinary PD excretion as well as rumen MN yield of TMR3 were significantly higher than those in TMR1 and TMR2 (P〈0.05). There was a significant positive relation between dietary RDP level and MN yield/digestible OM intake ( R^2=0.999 4,P=0.010 9) . The conversion rate of MCP was not affected by dietary RDP level ( P〉0.05) . The results indicate that under inferior forage feeding condition, when dietary RDP level is increased from 7. 94% to 10. 10%, nitrogen retention and rumen microbial protein ( MCP) synthesis can be improved, while the conversion rate of MCP is not affected.
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