高效液相色谱法同时测定染发剂中8种合成染料  

Simultaneous Determination of Eight Synthetic Dyes in Hair Dyes by High Performance Liquid Chromatography

作　　者：郑小严 欧阳立群 戴明 何树坤 黄红霞 林钦 周鹏 

机构地区：[1]国家加工食品质量监督检验中心福建省产品质量检验研究院,福建福州350002

出　　处：《分析测试学报》2015年第1期86-90,共5页Journal of Instrumental Analysis

基　　金：国家质检总局公益性行业科研专项项目(2012104013-5)

摘　　要：使用甲醇对样品进行超声提取,以乙腈-0.02 mol/L乙酸铵溶液为流动相,采用XB-C18色谱柱分离,二极管阵列检测器检测,外标法定量,建立了同时测定染发剂中8种合成染料的高效液相色谱分析方法。HC红3号、分散黑9、HC黄2号、分散紫1及HC橙1号的检测波长设为240 nm,HC黄4号设为410nm,HC蓝7号为450 nm,HC红1号为270 nm。8种染料在0.5-100.0μg/m L范围内具有良好线性,相关系数（r^2）均不小于0.999 3。HC红3号、HC黄4号、HC橙1号和分散紫1的检出限均为0.70 mg/kg,分散黑9、HC黄2号、HC蓝7号和HC红1号的检出限分别为2.50,0.60,0.35,0.50 mg/kg。样品的平均回收率为85.4%-99.8%,相对标准偏差（n=6）为1.1%-3.6%。方法的精密度、准确度、回收率和基质效应均符合染发剂样品测定的要求,用于实际样品测定,结果满意。该方法快速简便、定量准确,能满足染发剂中8种合成染料含量的分析检测要求。A high performance liquid chromatographic（HPLC） method was developed for the simultaneous detection of eight synthetic dyes in hair dyes.The samples were initially extracted with methanol,then separated on an XB-C18 column with acetonitrile and 0.02 mol/L ammonium acetate solution as mobile phases,and detected with DAD.The best detection wavelengths for HC Red No.3,HC Yellow No.4,HC Orange No.1,Disperse Violet 1,Disperse Black 9,HC Yellow No.2,HC Blue No.7 and HC Red No.1 are 240,410,240,240,240,240,450,270 nm,respectively.The external standard calibration curves were used for quantitative analysis.The calibration curves for 8 dyes were linear in the range of 0.5-100.0 μg/mL with correlation coefficients not less than 0.999 3.The limits of detection were 0.70 mg/kg for HC Red No.3,HC Yellow No.4,HC Orange No.1 and Disperse Violet 1,and 2.50,0.60,0.35 and 0.50 mg/kg for Disperse Black 9,HC Yellow No.2,HC Blue No.7 and HC Red No.1,respectively.The spiked recoveries were in the range of 85.4%-99.8% with relative standard deviations（RSDs,n=6） of 1.1%-3.6%.The precision,accuracy,mean recoveries and the matrix effects were satisfied for the requirements of cosmetic sample measurement.The proposed method was applied in the determination of 8 dyes in actual samples.The method is quick,easy,effective,sensitive and accurate,and could meet the requirements for the determination of eight synthetic dyes in hair dyes.

关 键 词：染发剂 合成染料 HC染料 分散染料 HPLC 

分 类 号：O657.72[理学—分析化学] F767.4[理学—化学]