反相高效液相色谱法测定冠心生脉丸中芍药苷含量  

Determination of Paeoniflorin Content in Guanxin Shengmai Pills by RP-HPLC

作　　者：张传平 吕紫璇 李洪斌 陈健 

机构地区：[1]重庆市涪陵食品药品检验所,重庆408000

出　　处：《中国药业》2015年第2期50-51,共2页China Pharmaceuticals

摘　　要：目的建立测定冠心生脉丸中芍药苷含量的反相高效液相色谱法。方法色谱柱为C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.05 mol/L磷酸二氢钾溶液（14∶86）,检测波长为230 nm,柱温为30℃。结果芍药苷进样量在0.106 0~1.695 3μg（r=0.999 4）范围内与峰面积呈良好线性关系,平均回收率为98.06%,RSD为0.93%（n=6）。结论该方法操作简便、快速、准确,可用于冠心生脉丸的质量控制。Objective To establish a RP-HPLC method for determining the content of paeoniflorin in Guanxin Shengmai Pills. Methods The RP-HPLC method was adopted anti the C18 eohmm（250 mm×4.6 mm,5μm） was used. The mohile phase was acetonitrile- 0.05 mol/L polassium dihydrogen phosphate（14 ：86）. The detection wavelength was 230 nm. The column temperature was 30 ℃. Results The sample size of paeoniflorin in the range 0. 106 0- 1. 695 3 μg（ r=0. 999 4） showed the good linear relation with the peak area. The average reeovery rate was 98.06%, RSD =0. 93% （n =6）. Conclusion This method is simple,rapid and accurate,which can be used for the quality control of Guanxin Shengmai Pills.

关 键 词：冠心生脉丸 芍药苷 含量 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]