STAT3蛋白在子宫内膜异位症合并不孕患者中的表达及临床意义  

Study on clinical significance of expression of STAT3 protein in patients with endometriosis associated with infertile

作　　者：王玲[1] 黄蓉[1] 许世艳[1] 刘新琼[1] 

机构地区：[1]广东省深圳市人民医院计划生育科,广东深圳518000

出　　处：《临床和实验医学杂志》2015年第2期104-106,共3页Journal of Clinical and Experimental Medicine

基　　金：广东省深圳市科技创新委员会;项目编号:JCYJ20140416122812010

摘　　要：目的研究STAT3蛋白在子宫内膜异位症合并不孕患者中的表达及临床意义。方法将2013年4月至2014年5月就诊的卵巢子宫内膜异位症合并不孕患者纳入研究对象,收集异位子宫内膜组织作为异位组,在位子宫内膜组织作为在位组;将同期就诊的子宫肌瘤患者纳入研究,收集子宫内膜组织作为对照组。采用实时聚合酶链反应和免疫印迹试验的方法检测子宫内膜组织中STAT3、p-STAT3、MMP2、MMP9的mRNA和蛋白含量。结果 1MMPs含量:异位组MMP2、MMP9的mRNA和蛋白含量高于在位组和对照组;2STAT3含量:三组间STAT3的mRNA和蛋白含量无差异,异位组p-STAT3的mRNA和蛋白含量高于在位组和对照组;3MMPs和STAT3的相关性:p-STAT3的蛋白含量与MMP2、MMP9的蛋白含量呈正相关。结论异位的子宫内膜组织中p-STAT3呈高表达,且可能通过促进MMP2、MMP9的表达来参与子宫内膜异位症的发生。Objective To explore the clinical significance of expression of STAT3 protein in patients with endometriosis associated with infertile. Methods Patients with ovarian endometriosis associated with infertile during April 2013 to May 2014 were enrolled,and eutopic endom-etrial tissues were collected and enrolled in eutopic group,tissues of ectopic endometrium were collected and enrolled in ectopic group;endometrial tissues of uterine fibroids in this hospital during the same period were collected and enrolled in control group. Then mRNA and protein content of STAT3,p - STAT3,MMP2 and MMP9 were detected by Real - time PCR and Western - blot. Results ① The content of MMPs,mRNA and protein content of MMP2 and MMP9 in ectopic group were higher than those of eutopic group and control group. ② STAT3 content：there is no sig-nificant difference in mRNA and protein content of STAT3 among three groups. mRNA and protein content of p - STAT3 in ectopic group were higher than those of eutopic group and control group. ③ Content of MMPs and STAT3：content of p - STAT3 protein was positively correlated with contents of MMP2 and MMP9 protein. Conclusion P - STAT3 is highly expressed in ectopic endometrial tissue and it may participate in the path-ogenesis of endometriosis by promoting the expression of MMP2 and MMP9.

关 键 词：子宫内膜异位症 信号转导和转录激活因子3 基质金属蛋白酶 

分 类 号：R711.71[医药卫生—妇产科学]