弱碱性蒽贝素氨水溶液小鼠尾静脉注射急性毒性实验研究  

Acute Toxicity Experimental Study on Slightly Alkaline Embelin and Ammonia Solution Administered as Tail Vein Injection in Mice

作　　者：曾宪彪 莫杰明 韦宝伟 韦桂宁 邓聿胤 

机构地区：[1]广西壮族自治区中医药研究院,广西南宁530022 [2]广西嘉盈生物科技有限公司,广西南宁530022

出　　处：《中国药物警戒》2015年第1期5-7,10,共4页Chinese Journal of Pharmacovigilance

基　　金：广西自然科学基金项目(2013GXNSFAA019261);广西医疗卫生重点科研课题(重2012057);广西中药质量标准研究重点实验室主任基金(桂中重科201103)

摘　　要：目的研究小鼠尾静脉注射弱碱性蒽贝素氨水溶液的急性毒性反应，测定经该途径给药后，小鼠半数致死剂量及其99％平均可信限。方法小鼠尾静脉注射弱碱性蒽贝素氨水溶液，预试出最大0％和最小100％致死剂量。再分别给12组小鼠尾静脉注射按1：0．8等比稀释的系列浓度溶液0．01mL·g^-1，剂量在最大0％致死剂量和最小100％致死剂量之间，观察给药后7日内各组小鼠中毒死亡情况。解剖检查死亡小鼠主要器官。计算半数致死剂量及其99％平均可信限。结果给药剂量在60～448mg·kg^-1间时，最低、最高剂量组小鼠死亡率介于30％和70％之间，剂量对数与死亡机率单位之间呈近似线性关系，可以用综合法计算半数致死剂量及其99％平均可信限。结论以蒽贝素量计，尾静脉注射弱碱性蒽贝素氨水溶液小鼠半数致死剂量为153．525mg·kg^-1，其99％平均可信限为153．525±40．815mg·kg^-1。Objective To study the acute toxicity reaction and to determine median lethal dose and its 99% confidence limit when mice received injection slightly alkaline embelin and ammonia solution from tail vein. Methods Mice received tail vein injection of slightly alkaline embelin and ammonia solution, pilot test was carried out to look for maximum 0% lethal dose and minimum 100% lethal dose. And then, twelve groups mice were injected slightly alkaline embelin and ammonia solution at 0.01 mL.g ^-1 from tail vein according to 1：0.8 geometric dilution, dose of each group was between minimum 0% lethal dose and maximum 100% lethal dose. Mice poisoning death in each group within 7 days after the treatment was observed. Dead mice were dissected to check primary organs. Median lethal dose and its 99% confidence limit were calculated. Results Mice mortality of the lowest dose group and the highest dose group was between 30% and 70%. There was approximate linear relationship between dosage logarithm and death probability unit, when tail vein injection dose was between 60 mg.kg^-1 and 448 mg.kg^-1. Median lethal dose and its 99% confidence limit were calculated by synthetic analyzing method. Conclusion With equivalent ofembelin, mice received tail vein injection of slightly alkaline confidence limit was 153.525±40 embelin and ammonia solution, median lethal dose was 153.525 mg.kg^-1 and its 99% 815 mg.kg^-1.

关 键 词：蒽贝素 弱碱性蒽贝素氨水溶液 小鼠 急性毒性 半数致死剂量. 

分 类 号：R992[医药卫生—毒理学] R28[医药卫生—药学]