细菌GYZC02株的鉴定及抗血栓的活性研究  

Identification of Bacteria Strain GYZC02 and Study on Its Antithrombotic Active Substances

作　　者：张健[1] 李锵[1] 王伦兴 王召贺 杨再昌[1,2] 

机构地区：[1]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学药学院,贵州贵阳550025

出　　处：《安徽农业科学》2015年第2期120-122,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省社会发展科技攻关项目(SY字[2013]3058号);国家自然科学基金(81460531)

摘　　要：[目的]鉴定细菌GYZC02株,并研究其抗血栓活性。[方法]利用形态学特征、生化试验和16S rRNA序列同源性比较鉴定菌株,通过小鼠断尾试验和小鼠尾动脉血栓模型验证GYZC02发酵液的抗血栓活性。[结果]根据形态学、生化试验和16S r DNA序列测定结果,该菌株鉴定为枯草芽孢杆菌。通过灌胃给药发现,GYZC02发酵液乙酸乙酯萃取物能明显延长小鼠断尾出血时间,抑制小鼠尾动脉血栓的形成,表明该细菌具有抗血栓活性。[结论]枯草芽孢杆菌GYZC02发酵液的小分子物质具有抗血栓活性。[ Objective ] To identify bacteria strain of GYZC02 and study its antithrombotic activity. [ Method ] Morphological characteristic, bio- chemical test, and 16S rRNA sequence homology were used to identification of GYZC02 strain. The mouse tail bleeding time and arterial throm- bosis model were used to assay the antithrombotic activity of GYZC02. [ Resultl The results of morphological, biochemical test, and 16S rRNA sequences revealed that the strains is a kind of Bacillus subtilis. By oral administration, the ethyl acetate extract GYZC02 broth could significantly prolong the bleeding time, and inhibit the formation of mouse tail artery thrombosis. [ Conclusion] Bacillus subtilis GYZC02 can produce small molecules with high antithrombotic activity.

关 键 词：GYZC02 鉴定 抗血栓活性 

分 类 号：S852.6[农业科学—基础兽医学]