IFN-γ与IL-4检测在小儿哮喘及肺炎中的临床应用  

The clinical application of IFN-γ and IL-4 in children with asthma and pneumonia

作　　者：张虹[1] 顾猛[2] 

机构地区：[1]江苏省南通瑞慈医院检验科,江苏南通226009 [2]江苏省南通大学附属常州儿童医院检验科,江苏常州213003

出　　处：《转化医学电子杂志》2014年第6期56-57,共2页E-Journal of Translational Medicine

基　　金：南通市科技项目（HS2011060）

摘　　要：目的：探索IFN-γ与IL-4检测在小儿哮喘及肺炎中的临床应用．方法：用ELISA法检测282例小儿哮喘、306例小儿肺炎及同期幼儿园和小学生健康体检者250例小儿血清中IFN-γ与IL-4水平，结合临床相关检查资料，对检测结果采用SPSS18．0统计软件进行统计学分析，各组实验数据以x ±s表示，各实验组间比较计量资料的t检验，P＜0．05时认为差异有统计学意义．结果：哮喘组患儿外周血IFN-γ[（21．4±31．7）ng／L]明显低于肺炎组[（215．8±43．1）ng／L]及对照组[（276．7±43．2）ng／L]，而肺炎组[（245．8±43．1）ng／L]与对照组[（276．7±43．2）ng／L]之间没有显著性差异；哮喘组IL-4的水平[（12．6±7．9）ng／L]明显高于肺炎组[（5．1±3．7）ng／L]及对照组[（4．6±3．7）ng／L]，而肺炎组[（5．1±3．7）ng／L]与对照组[（4．6±3．7）ng／L]之间没有显著性差异．结论：哮喘患儿存在着明显的免疫失衡问题，说明IFN-γ与IL-4具有协同作用，从而导致Th1／Th2平衡失调，这可能是导致小儿哮喘的主要原因之一，在治疗时应着重于免疫调理；而肺炎患儿Th 1／Th2变化不大，说明其发病机理主要是因为炎症等其它因素所致，在治疗时应着重于消炎．AIM：To explore the clinical application of IFN-γ and IL-4 in children with asthma and pneumonia.METHODS：The levels of IFN-γand IL-4 of 282 cases of children with asth-ma,306 cases with pneumonia and 250 health students as control group were detected by ELISA at the same time.The clinical relative factors and experimental data were statistically analyzed using SPSS18.0 statistical software,each set of experimental data ex-pressed as x ±s deviation,in each experimental group were ana-lyzed using t-test,P〈0.05 was considered statistically signifi-cant.RESULTS：The levels of IFN-γ[（21.4 ±31.7）ng/L]of asthmatic children were remarkably lower than those in the pneu-monia group [（245.8 ±43.1 ） ng/L ] and control group [（276.7 ±43.2）ng/L],P〈0.01,while the experimental data between the pneumonia group and control group have no remarka-ble statistically significant difference,P〉0.05.The levels of IL-4 [（12.6 ±7.9）ng/L]of asthmatic children were remarkably higher than those in the pneumonia group [（5.1 ±3.7）ng/L] and control group [（4.6 ±3.7）ng/L],P〈0.01,while the experimental data between the pneumonia group and control group have no remarkable statistically significant difference either,P〉0.05.CONCLUSION：Children with asthma existing obvious immune imbalance indicate IFN-γand IL-4 have a synergistic effect,which leads to the immune imbalance of Th1/Th2 and this may be the main cause of asthma in children.We must emphasize immune regulation during the treatment.While Th1/Th2 has little change in children with pneumonia,this indicates the pathogenesis of pneumonia is mainly due to other factors such as inflamma-tion and we must emphasize diminishing inflammation during the treatment.

关 键 词：哮喘 肺炎 IFN-Γ IL-4 

分 类 号：R725.6[医药卫生—儿科]