黑龙江省大豆花叶病毒(SMV)株系的动态变化分析  

Analysis of Dynamic Change of Soybean Mosaic Virus Strains in Heilongjiang Province of China

作　　者：李凯[1] 夏迎春[1] 王大刚[1] 杨永庆[1] 任锐[1] 高乐[1] 张锴[1] 智海剑[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《大豆科学》2014年第6期880-884,共5页Soybean Science

基　　金：国家自然科学基金(31101164;31171574);现代农业产业技术体系(CARS-004);转基因生物新品种培育科技重大专项(2008ZX08004-004);"十二五"农村领域国家科技计划(2011BAD35B06-4)

摘　　要：2012年大豆花叶病毒(SMV)病发病盛期,在我国黑龙江省大豆主产区的哈尔滨、绥化、黑河、齐齐哈尔、佳木斯和牡丹江市的68块大豆生产田、国营农场及部分科研单位的试验田采集病样461份,经在感病大豆品种南农1138-2上初步繁殖鉴定、单斑分离纯化和血清学鉴定,最终获得阳性SMV单一分离物63个。采用一套共10个大豆鉴别寄主鉴定了63个分离物在鉴别寄主上的反应,发现63个分离物分属2个SMV致病型。其中6个(9.5%)分离物属SC15株系,57个(90.5%)分离物属SC18株系。发现黑龙江省SMV株系组成较简单,强毒株系SC15零星发生,弱毒株系SC18为优势株系且分布较广。与以往鉴定结果比较,发现SC15与吕文清等鉴定的东北3号株系致病性相近,SC18与东北1号株系相近。建议黑龙江省大豆SMV防控和大豆抗病育种应以优势SMV株系SC18为主,并兼顾强毒株系SC15。From 461 leaf samples with soybean mosaic virus（ SMV）-like symptoms collected from 68 fields（ production fields,some experimental farm and research plots） of 6 cities（ Harbin,Suihua,Heihe,Qiqihar,Jiamusi and Mudanjiang） of Heilongjiang province in China during soybean mosaic virus disease onset peak in 2012,sixty-three positive SMV isolates were obtained after preliminary identification on the susceptible soybean cv. Nannong 1138-2,biological purification with single-lesion isolation and serological determination. Based on disease reactions on 10 differentials used in China as a representative from various reported differential cultivar systems,the 63 isolates were divided into 2 strains. Six isolates were classified into strain SC15,and the other 57 isolates were SC18 strain. The composition of SMV strains found in Heilongjiang province was relatively simple,less virulent strain SC18,which was predominant and widespread in Heilongjiang province,accounted for 90. 5% of the total isolates and was distributed in 6 cities. While virulent strain SC15,accounted for only 9. 5% of the total isolates and was distributed in 3 cities. Comparison with the previous identification results showed that SC15 and SC18 were similar to Northeastern No. 3 strain and No. 1 strain identified by Lyu et al in pathotypic,respectively. It is clear that SC18 is the key consideration SMV strains during disease control and soybean breeding program for SMV resistance.

关 键 词：大豆 大豆花叶病毒 单斑分离生物学纯化 血清学鉴定 鉴别寄主 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]