离子交换色谱法测定葡萄糖氧化反应液中的葡萄糖和葡萄糖酸  

Determination of Glucose and Gluconate in Oxidation Reaction Aqua by Anion-Exchange Chromatography

作　　者：王鑫[1,2] 梁文辉[2,3] 刘颖慧[2,3] 赵富华[2] 法芸[2] 

机构地区：[1]青岛大学化学科学与工程学院,山东青岛266071 [2]中国科学院青岛生物能源与过程研究所,生物基材料重点实验室,山东青岛266101 [3]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《化学分析计量》2014年第B12期5-8,共4页Chemical Analysis And Meterage

基　　金：国家自然科学基金项目(21405166);科技部重大科学仪器开发专项任务(2012YQ09022908);山东省科学仪器升级改造项目(2012SJGZ12)

摘　　要：建立了一种利用阴离子交换色谱分离,以积分脉冲安培检测器同时测定葡萄糖氧化反应液中葡萄糖和葡萄糖酸的方法。采用Ion Pac AS11–HC阴离子交换柱,以5.0 mmol/L KOH溶液作为淋洗液,等度洗脱,流量为1.0m L/min,柱温为30℃,进样体积为25μL。葡萄糖和葡萄糖酸测定结果的相对标准偏差分别为0.66%,1.32%(n=6),线性相关系数分别为0.989 3,0.992 9,平均加标回收率分别为99.50%,109.0%。该方法可用于葡萄糖氧化反应液中葡萄糖和葡萄糖酸的同时测定。Simultaneous analysis of glucose and gluconate in oxidation reaction aqua was presented by using anionexchangechromatography and pulsed amperometric detection. Separation was performed on an IonPac AS11–HC columnwith 1.0 mL/min eluent of 5.0 mmol/L KOH solution. The injection volume was 25μL and the column temperaturewas 30℃ . The relative standard derivations of glucose and gluconate were 0.66%,1.32% （n=6）, and the linear correlationcoefficent were 0.989 3,0.992 9, respectively. The average recoveries were 99.50% and 109.0%, respectively. This methodcan be applied to determine glucose and gluconate in oxidation reaction aqua with satisfactory results.

关 键 词：阴离子交换色谱 脉冲安培检测 葡萄糖 葡萄糖酸 

分 类 号：O657.7[理学—分析化学]