高效阴离子交换色谱积分脉冲安培法测定蓝藻细胞培养液中的蔗糖和甘油葡糖苷  

Measurement of Sucrose and Glucosylglycerol in Fermentation of Cyanobacterial by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

作　　者：梁文辉[1,2] 法芸[2] 王明林[1] 

机构地区：[1]山东农业大学,食品科学与工程学院,山东泰安271018 [2]中国科学院青岛生物能源与过程研究所,生物燃料重点实验室,山东青岛266101

出　　处：《化学分析计量》2014年第B12期9-12,共4页Chemical Analysis And Meterage

基　　金：国家自然科学基金项目(21405166);科技部重大科学仪器开发专项任务(2012YQ09022908);山东省科学仪器升级改造项目(2012SJGZ12)

摘　　要：建立了一种测定蓝藻细胞培养液中蔗糖和甘油葡糖苷的高效阴离子交换色谱积分脉冲安培检测法.在最佳的分离条件下,蔗糖和甘油葡糖苷的质量浓度在0.1～50.0mg/L 范围内与色谱峰面积线性良好,线性相关系数r2〉0.999 9.蔗糖和甘油葡糖苷的最低检测限分别是0.15mg/L 和0.03mg/L,测定结果的相对标准偏差小于2%（n=8）.该方法样品处理简单,无基体干扰,测定的准确度,灵敏度高,可应用于蓝藻培养液中蔗糖和甘油葡糖苷的测定.A high performance anion-exchange chromatography with pulsed amperometric detection was developed forthe determination of sucrose and glucosylglycerol in cyanobacterial cells. Under the optimized conditions,the concentrationof sucrose and glucosylglycerol showed good linearity （r2〉0.999 9） with the chromatographic peak area in the range of0.1–50.0 mg/L. The limit of detection（LOD） was 0.15 mg/L for sucrose and 0.03 mg/L for glucosylglycerol. RSDs ofthe detection results were less than 2% （n=8）. The result showed that the method was convenient and accurate for thedetermination of sucrose and glucosylglycerol in cyanobacterial cells.

关 键 词：高效阴离子交换色谱 积分脉冲安培检测 蓝藻 培养液 蔗糖 甘油葡糖苷 

分 类 号：O657.7[理学—分析化学]