Effects of Temperature, Acetate and Nitrate on Methane Generation from Petroleum Hydrocarbons  

作　　者：Liu Chunshuang Zhao Dongfeng Zhang Yunbo 

机构地区：[1]College of Chemical Engineering, China University of Petroleum,Qingdao

出　　处：《China Petroleum Processing & Petrochemical Technology》2014年第4期24-31,共8页中国炼油与石油化工（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.21307160);the Natural Science Foundation of Shandong Province(No.ZR2013EEQ030);the Fundamental Research Funds for the Central Universities(No.24720142053A);the Research&Technology Development Project of China National Petroleum Corporation(No.2008D-4704-2)

摘　　要：In this study, the effects of temperature, acetate and nitrate on methane gas production from biodegradation of petroleum hydrocarbons were investigated. The results indicated that methane gas production at 35 ℃ was higher than that obtained at 55 ℃. The acetate addition significant enhanced the methane production at 35 ℃, however, at 55 ℃ the nitrate addition could largely promote the methane production. The microbial community structures were revealed by PCR-DGGE. The Actinobacteria, Clostridia, Clostridiales, Syntrophus, Pseudomonas, and Proteobacteria-like bacteria and Methanocellales, Methanosaeta, Methanomicrobiales, Methanolinea, Thermoprotei-like archaea had been enriched at 35 ℃ in the acetate addition test. The Thermoprotei, Proteobacterium, Thermodesulfovibrio-like bacteria and Methanocellales-like archaea had been enriched at 55 ℃ in the nitrate addition test. The results may shed light on the bio-utilization of marginal oil reservoirs for enhancing energy recovery.In this study, the effects of temperature, acetate and nitrate on methane gas production from biodegradation ofpetroleum hydrocarbons were investigated. The results indicated that methane gas production at 35 ℃ was higher than thatobtained at 55 ℃. The acetate addition significant enhanced the methane production at 35 ℃, however, at 55 ℃ the nitrateaddition could largely promote the methane production. The microbial community structures were revealed by PCR-DGGE.The Actinobacteria, Clostridia, Clostridiales, Syntrophus, Pseudomonas, and Proteobacteria-like bacteria and Methanocellales,Methanosaeta, Methanomicrobiales, Methanolinea, Thermoprotei-like archaea had been enriched at 35 ℃ in theacetate addition test. The Thermoprotei, Proteobacterium, Thermodesulfovibrio-like bacteria and Methanocellales-like archaeahad been enriched at 55 ℃ in the nitrate addition test. The results may shed light on the bio-utilization of marginal oilreservoirs for enhancing energy recovery.

关 键 词：PETROLEUM hydrocarbons NITRATE METHANE MICROBIAL community 

分 类 号：TE626[石油与天然气工程—油气加工工程]