丝网印刷电极法测定血铅的方法学评价  

Methodology evaluation on the determination of blood lead by screen printing electrode method

作　　者：余晓刚 颜崇淮[1] 盛青松 

机构地区：[1]上海交通大学医学院附属新华医院上海市环境与儿童健康重点实验室,上海200092 [2]无锡市申瑞生物制品有限公司,江苏无锡214092

出　　处：《检验医学》2014年第12期1230-1233,共4页Laboratory Medicine

基　　金：科技部973项目(2012CB525001);环境保护部行业公益性项目(201309048);江苏省科技支持项目(BE2012628;BK2012539)

摘　　要：目的比较丝网印刷电极(SPE)法和石墨炉原子吸收光谱(GFAAS)法在血铅检测中的应用,评价SPE法的实用性和可靠性。方法采用SPE法检测卫生部临床检验中心室间质控样本和全血样本,进行方法学评价,并与GFAAS法进行比较。结果 SPE法检测血铅低、中、高3个水平样本的相对标准偏差(RSD)分别为14.80%、4.03%、2.11%,平均回收率为103.7%。SPE法测定全血样本的结果与GFAAS法比较差异无统计学意义(P>0.05),线性回归分析相关系数(r2)为0.964,2种方法呈高度相关。Bland-Altman分析显示2种方法具有一致性。以GFAAS法为金标准,SPE法假阳性率为4.4%,假阴性率为0.0%。结论 SPE法稳定性强、操作简便,与GFAAS法呈高度相关,适用于临床大批量样本的快速检验。Objective To compare the applications of screen printing electrode( SPE) method and graphite furnace atomic absorption spectrophotometry( GFAAS) for blood lead determination,and to evaluate the practicability and reliability of SPE method. Methods The SPE method was used to detect the quality control sample of the National Center for Clinical Laboratory and whole blood samples in order to evaluate methodologically,and the results were compared with those of GFAAS. Results The SPE method was used to determine high,medium and low levels of blood lead,and the relative standard deviation( RSD) were 14. 80%,4. 03% and 2. 11%,respectively. The average recovery rate of SPE method was 103. 7%. When SPE method was used to the determination of whole blood samples,there was no statistical significance compared with GFAAS( P > 0. 05). The linear regression correlation coefficient( r2) was 0. 964. There was a high correlation between the 2 methods. The Bland-Altman analysis showed that the 2methods had consistency. With GFAAS as gold standard,the false positive rate of SPE method was 4. 4%,and the false negative rate was 0. 0%. Conclusions The SPE method shows a marked performance in stability and ease of operation,and it is highly correlated with GFAAS,which is suitable for clinical bulk samples' rapid determination.

关 键 词：血铅 丝网印刷电极法 石墨炉原子吸收光谱法 

分 类 号：R446.1[医药卫生—诊断学]