RP-HPLC法测定清鱼油霜中维生素A含量  

Content Determination of Vitamin A in Cod Liver Oil Cream by RP- HPLC

作　　者：聂秀霞 何婉敏 陈焕梅 邱玉婷 

机构地区：[1]中山市第二人民医院,广东中山528447

出　　处：《江西中医药大学学报》2014年第6期72-73,76,共3页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的:建立RP-HPLC法测定清鱼油霜中维生素A含量的方法,为清鱼油霜的质量控制提供依据。方法:采用Eclipse XDB-C18(4.6mm×150mm,5μm)色谱柱,流动相:甲醇-水=95∶5,检测波长:325nm,柱温:30℃。结果:维生素A浓度在3.07μg/m L^30.74μg/m L之间时,与峰面积呈良好的线性关系,回归方程为Y=119.17X+0.8525,r=1,平均回收率为101.39%,RSD为3.01%。结论:此方法简便可行,可以作为测定清鱼油霜中维生素A含量的测定方法。Objective ： To establish a method to determine the content of vitamin A in pure fish oil cream by RP - HPLC and to provide the basis for the quality control of clear fish oil cream. Methods： Using Eclipse XDB - C18 （4.6 mm × 150mm, 5 μm） , Methanol - Wa- ter = 95：5 as mobile phase and detection wavelength of 325 nm. Results： Vitamin A concentration in 3. 07μg/mL -30. 74μg/mL, had good linear relationship with the peak area, and the regression equation for Y = 119.17X ＋ 0.8525, r = 1, the average recovery was 101.39% , RSD was 3.01%. Conclusion： This method is simple and feasible, and can be used as the method to determine the content of vitamin A in pure fish oil cream .

关 键 词：清鱼油霜 HPLC 维生素A 

分 类 号：R927.2[医药卫生—药学]