星点设计-响应面法优化续断总皂苷提取工艺  被引量:4

Optimization of Extraction Technology for Total Saponins in Dipsaci Radix by Central Composite Design-Response Surface Methodology

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作  者:葛庆[1] 范茜茜[1] 彭翠香[1] 许汉林[1] 

机构地区:[1]湖北中医药大学药学院,武汉430065

出  处:《世界科学技术-中医药现代化》2014年第11期2395-2399,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基  金:科学技术部国家重大新药创制重大科技专项(2014ZX09304-307-001):中药新药安全性检测技术与标准研究-中药原料药混伪品分子基因鉴定技术与标准;主课题负责人:肖红斌;子课题(2014ZX09304-307-001-021)负责人:胡志刚

摘  要:目的:优选续断总皂苷的提取工艺。方法:以单因素实验为基础,选择提取浓度、提取时间和溶剂用量为自变量,以续断总皂苷含量和续断皂苷Ⅵ含量的"总评归一值"为响应值,采用星点设计-响应面法对数据进行多元线性和二项式拟合,应用响应面法优选续断总皂苷的提取工艺并对最佳工艺预测分析。结果:二项式拟合方程(R2=0.964 2)优于多元线性回归方程(R2=0.663 0),确定最佳提取工艺为:7倍量55%乙醇回流提取3次,每次2.8 h。经最佳提取工艺提取得到续断总皂苷含量为0.201 1 g·g-1,与预测值偏差1.83%;续断皂苷Ⅵ含量为0.015 4 g·g-1,与预测值偏差3.37%。结论:星点设计-响应面法优化的提取工艺简便可行,且预测性良好,为续断总皂苷的进一步开发利用提供了依据。To optimize the extraction technology of the total saponins in Dipsaci Radix, the extraction efficiency of total saponins was investigated with respecting to three variables including time, ethanol concentration and liquid-to-solid ratio. On the basis of a series of one-factor-at-a-time experiments, a polynomial regression model equation was fitted by the combined use of central composite experimental design and regression analysis. By analyzing the regression model using response surface analysis, the optimum extraction conditions of total saponins from Dipsaci Radix were identified as follows: 7 times of 55% alcohol, refluxing 3 times, 2.8 hours every time, and the extraction efficiency of total saponins was up to 0.201 1 g·g^-1 and the asperosaponin Ⅵ was up to 0.015 4 g·g^-1 under the optimized conditions. Confirmatory experiment indicated the good prediction ability of the established model and provided the basis for further development and utilization of Dipsaci Radix.

关 键 词:续断 总皂苷 响应面法 提取工艺 

分 类 号:R284.2[医药卫生—中药学]

 

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