针刺穴位与筋膜对骨髓间充质干细胞定向分化的体外机制研究  

Research on Mesenchymal Stem Cells Differentiation by Serum from Hepatic Fibrosis Rats by Acupuncture on Acupoint and Connective Tissues in Vitro

作　　者：张玮[1] 郑志忠[1] 赵福建[1] 张铭[1] 赵丽云[1] 郭顺根[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界科学技术-中医药现代化》2014年第11期2400-2405,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重点基础研究发展计划"973计划"项目(2007CB512705):中医经络的筋膜学基础研究;负责人:原林

摘　　要：目的:探讨针刺穴位与筋膜在治疗肝纤维化中的体外机制。方法:大鼠分为正常对照组、肝纤维化模型12周组、肝纤维化模型20周组、针刺穴位组和针刺筋膜组。采用HE染色和Mallory染色,光镜下观察各组大鼠肝脏石蜡切片标本,制备各组血清并检测血清中的血清甘油三酯(TG)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ),验证模型是否造模成功,针刺穴位组和针刺筋膜组是否有效,同时制备各组大鼠血清,对大鼠骨髓间充质干细胞进行干预,以肝细胞标志物白蛋白作为检测指标,采用免疫组化、ELISA法和免疫印迹法检测各组血清对大鼠骨髓间充质干细胞分化增殖是否具有影响。结果:HE染色和Mallory染色及血清学指标显示,造模12周后,大鼠肝脏出现了明显的纤维化和肝细胞的脂肪变,模型组血清中TG、HA、LN、PC-Ⅲ浓度明显高于其他各组。造模20周后,大鼠肝脏出现一定程度自愈,但效果明显差于两个治疗组。免疫组化法、ELISA法和免疫印迹法显示正常对照组血清干预的大鼠骨髓间充质干细胞无白蛋白表达,20周模型组、针刺穴位组和针刺筋膜组干预的大鼠骨髓间充质干细胞均有白蛋白表达。结论:正常对照组血清不能诱导骨髓间充质干细胞向肝细胞分化;而20周模型组、针刺穴位组和针刺筋膜组大鼠血清可诱导骨髓间充质干细胞向肝细胞分化;这提示损伤及治疗均可诱导骨髓间充质干细胞向肝细胞分化。This study was aimed to discuss cellular mechanism of anti-liver fibrosis by acupuncture on acupoint and connective tissues. Rats were divided into five groups, which were the normal group, liver fibrosis model group （12 week）, liver fibrosis model group（20 week）, acupoint acupuncture group, and connective tissue acupuncture group. The observation was made on morphology of liver in various groups by HE stain and Mallory stain. Concentrations of TG, HA, LN and PC-Ⅲin serum were also detected. Mesenchymal stem cells （MSCs） were intervened by serum from each group to observe MSCs proliferation and differentiation. The results showed that the serum level of model group（12 week） was significantly different from other groups. And the same results were found in the inspection of morphology. In the normal group there were no cell expressing albumin. In other groups, MSCs can express albumin. It was concluded that the serum of normal group cannot make MSCs transform into hepatocyte-like cell. The serum of other groups can make MSCs transform into hepatocyte-like cell. It was concluded that both hepatic fibrosis and acupuncture can induce MSCs differentiation.

关 键 词：肝纤维化 骨髓间充质干细胞 针刺穴位 针刺筋膜 

分 类 号：R245[医药卫生—针灸推拿学]